Document Detail


Establishment and characterization of a human cell line from a squamous carcinoma of the tongue.
MedLine Citation:
PMID:  7788927     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A human squamous carcinoma cell line, PWH-S1, has been established from the metastatic lymph node of a Chinese patient with a squamous cell carcinoma of the tongue. The fibroblast-free culture has been propagated in DMEM supplemented with 10% fetal bovine serum for more than 100 passages. PWH-S1 cells showed anchorage-independent growth in 0.3% agar solution. PWH-S1 cell line exhibited a monolayer growth and loss of contact inhibition. The in vitro doubling time of the PWH-S1 cell line was approximately 28 h. PWH-S1 was tumourigenic after inoculation into nude mice. Karyotype analysis showed the chromosome abnormality with a modal number of 69. Electron microscopy demonstrated poorly differentiated neoplastic cells with some features of squamous cell differentiation. No human papillomavirus (type 6, 11, 16, 18, 31) was detected in the PWH-S1 cell line by the polymerase chain reaction.
Authors:
W W King; P K Lam; D W Huang; C T Liew; A K Li
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Clinical otolaryngology and allied sciences     Volume:  20     ISSN:  0307-7772     ISO Abbreviation:  Clin Otolaryngol Allied Sci     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-07-27     Completed Date:  1995-07-27     Revised Date:  2005-05-02    
Medline Journal Info:
Nlm Unique ID:  7701793     Medline TA:  Clin Otolaryngol Allied Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  15-20     Citation Subset:  IM    
Affiliation:
Department of Surgery, Prince of Wales Hospital, Chinese University of Hong Kong.
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MeSH Terms
Descriptor/Qualifier:
Adult
Carcinoma, Squamous Cell / pathology*,  ultrastructure
Cells, Cultured
Chromosomes
Culture Techniques
DNA Probes, HPV
Female
Humans
Karyotyping
Microscopy, Electron
Polymerase Chain Reaction
Tongue / pathology*,  ultrastructure
Tongue Neoplasms / pathology*,  ultrastructure
Chemical
Reg. No./Substance:
0/DNA Probes, HPV

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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