| Establishment and characterization of a goat synovial membrane cell line susceptible to small ruminant lentivirus infection. | |
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MedLine Citation:
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PMID: 15081607 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Primary goat synovial membrane (GSM) cells are widely used to study small ruminant lentiviruses (SRLV), i.e. maedi visna virus (MVV) and caprine arthritis-encephalitis virus (CAEV), but their limited life-span of 15-20 passages in vitro is problematic. Here, we report that ectopic expression of the catalytic subunit of human telomerase (hTERT) was sufficient to immortalize primary GSM cells. Cultures of hTERT-transfected GSM cells have been passaged for 2 years without showing any phenotypic difference from the original primary GSM cells. The hTERT-transfected cells continued to grow beyond a population doubling number of 250, while no net telomere lengthening was observed for these cells. Moreover, the immortalized GSM cells were susceptible to infection by both CAEV and MVV and were able to propagate theses viruses. Such cell line provides a useful source of standard and robust cells for both research and veterinary purposes. |
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Authors:
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Morgane Rolland; Cécile Chauvineau; Stephen Valas; Robert Z Mamoun; Gérard Perrin |
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Publication Detail:
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Type: Evaluation Studies; Journal Article |
Journal Detail:
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Title: Journal of virological methods Volume: 118 ISSN: 0166-0934 ISO Abbreviation: J. Virol. Methods Publication Date: 2004 Jun |
Date Detail:
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Created Date: 2004-04-14 Completed Date: 2004-06-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8005839 Medline TA: J Virol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 123-30 Citation Subset: IM |
Affiliation:
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AFSSA-Niort, Laboratoire d'Etudes et de Recherches Caprines, 79012 Niort, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arthritis-Encephalitis Virus, Caprine / physiology* Cell Division Cell Line Goats* Humans Synovial Membrane / virology* T-Lymphocytes / physiology Telomerase / genetics Transfection Virology / methods Visna-maedi virus / physiology* |
| Chemical | |
Reg. No./Substance:
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EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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