Document Detail

Establishment and characterization of a fibroblast cell line from the Mongolian horse.
MedLine Citation:
PMID:  19263179     Owner:  NLM     Status:  MEDLINE    
A fibroblast line was successfully established from Mongolian horse ear marginal tissue by using a primary explant technique and cell cryogenic preservation technology. Biological analysis showed the following: The cells were adherent and exhibited density-dependent inhibition of proliferation; assays of microbial contamination from bacteria, fungi, and mycoplasma were negative; the population doubling time of the cells was 33.9 h; and a 2n chromosome number of 64 at a frequency higher than 80%. A lack of cross-contamination of this cell line with other species was confirmed by isoenzyme analysis of lactic and malic dehydrogenases. In order to study exogenous gene expression, four fluorescent proteins, pEGFP-N3, pEGFP-C1, pDsRed1-N1, and pEYFP-N1, were transfected into the cells. The corresponding fluorescence was distributed throughout the cytoplasm and nucleus 12 h after transfection. This cell line not only preserves the genetic resources of the Mongolian horse at the cellular level but also provides valuable materials for genomic, postgenomic, and somacloning research in this species.
Lin-feng Li; Wei-jun Guan; Yue Hua; Xiu-juan Bai; Yue-hui Ma
Related Documents :
22354149 - A disulfide bound-molecular beacon as a fluorescent probe for the detection of reduced ...
10077219 - Establishment and characterization of a second primary osteosarcoma cell line (osrb/n-m...
22297039 - Assessment of the infiltration of inflammatory cells in the walls of thrombotic varicos...
6604099 - Alternative pathway of complement activation by human lymphoblastoid b and t cell lines.
7960959 - Quantitative and biochemical characterization of soluble hla class i antigens shed by c...
3756929 - In vitro differentiation and progression of mouse mammary tumor cells.
7729499 - Estradiol-induced changes in tsh-like immunoreactivity of pituitary cells in female rats.
15522409 - The iron chelating cardioprotective prodrug dexrazoxane does not affect the cell growth...
17822359 - Design of tissue engineering scaffolds as delivery devices for mechanical and mechanica...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-05
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  45     ISSN:  1543-706X     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:    2009 Jul-Aug
Date Detail:
Created Date:  2009-07-23     Completed Date:  2009-11-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  311-6     Citation Subset:  IM    
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Adhesion
Cell Culture Techniques
Cell Line*
Cell Proliferation
Fibroblasts / cytology*
Horses / genetics*
Luminescent Proteins / analysis,  genetics
Reg. No./Substance:
0/Luminescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Lectin binding patterns reflect the phenotypic status of in vitro chondrocyte models.
Next Document:  Laparoscopic placement of non-adjustable silicone ring for weight regain after Roux-en-Y gastric byp...