Document Detail


Establishment and characterization of a fibroblast cell line from the Mongolian horse.
MedLine Citation:
PMID:  19263179     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A fibroblast line was successfully established from Mongolian horse ear marginal tissue by using a primary explant technique and cell cryogenic preservation technology. Biological analysis showed the following: The cells were adherent and exhibited density-dependent inhibition of proliferation; assays of microbial contamination from bacteria, fungi, and mycoplasma were negative; the population doubling time of the cells was 33.9 h; and a 2n chromosome number of 64 at a frequency higher than 80%. A lack of cross-contamination of this cell line with other species was confirmed by isoenzyme analysis of lactic and malic dehydrogenases. In order to study exogenous gene expression, four fluorescent proteins, pEGFP-N3, pEGFP-C1, pDsRed1-N1, and pEYFP-N1, were transfected into the cells. The corresponding fluorescence was distributed throughout the cytoplasm and nucleus 12 h after transfection. This cell line not only preserves the genetic resources of the Mongolian horse at the cellular level but also provides valuable materials for genomic, postgenomic, and somacloning research in this species.
Authors:
Lin-feng Li; Wei-jun Guan; Yue Hua; Xiu-juan Bai; Yue-hui Ma
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-05
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  45     ISSN:  1543-706X     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:    2009 Jul-Aug
Date Detail:
Created Date:  2009-07-23     Completed Date:  2009-11-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  311-6     Citation Subset:  IM    
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China. lilinfeng219@126.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion
Cell Culture Techniques
Cell Line*
Cell Proliferation
Cryopreservation
Fibroblasts / cytology*
Horses / genetics*
Karyotyping
Luminescent Proteins / analysis,  genetics
Chemical
Reg. No./Substance:
0/Luminescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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