Document Detail


Establishment and characterization of embryonic stem-like cells from porcine somatic cell nuclear transfer blastocysts.
MedLine Citation:
PMID:  20307349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was aimed to establish embryonic stem (ES)-like cells from blastocysts derived from somatic cell nuclear transfer (SCNT) in pig. Somatic cells isolated from both day-30 fetus and neonatal cloned piglet were used for donor cells. A total of 60 blastocysts (46 and 14 derived from fetal and neonatal fibroblast donor cells, respectively) were seeded onto a mitotically inactive mouse embryonic fibroblast (MEF) monolayer and two ES-like cell lines, one from each donor cell type, were established. They remained undifferentiated over more than 52 (fetal fibroblast-derived) and 48 (neonatal fibroblast-derived) passages, while retaining alkaline phosphatase activity and reactivity with ES specific markers Oct-4, stage-specific embryonic antigen-1 (SSEA-1), SSEA-4, TRA-1-60 and TRA-1-81. These ES-like cells maintained normal diploid karyotype throughout subculture and successfully differentiated into embryoid bodies that expressed three germ layer-specific genes (ectoderm: beta-III tubulin; endoderm: amylase; and mesoderm: enolase) after culture in leukemia inhibitory factor-free medium. Microsatellite analysis confirmed that they were genetically identical to its donor cells. Combined with gene targeting, our results may contribute to developing an efficient method for producing transgenic pigs for various purposes.
Authors:
S Kim; J H Kim; E Lee; Y W Jeong; M S Hossein; S M Park; S W Park; J Y Lee; Y I Jeong; H S Kim; Y W Kim; S H Hyun; W S Hwang
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Publication Detail:
Type:  Journal Article     Date:  2010-03-22
Journal Detail:
Title:  Zygote (Cambridge, England)     Volume:  18     ISSN:  1469-8730     ISO Abbreviation:  Zygote     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-07     Completed Date:  2010-05-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9309124     Medline TA:  Zygote     Country:  England    
Other Details:
Languages:  eng     Pagination:  93-101     Citation Subset:  IM    
Affiliation:
SooAm Biotech Research Foundation, Wonsam-myeon, Cheoin-gu, Yongin-si, Gyeonggi-do, 449-872, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn*
Blastocyst / cytology*
Embryo, Mammalian / cytology
Embryonic Stem Cells / cytology
Female
Fetus / cytology*
Fibroblasts / cytology
Mice
Nuclear Transfer Techniques*
Swine
Swine, Miniature* / embryology

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