Document Detail

Establishment and characterization of conditionally immortalized organ of corti cell lines.
MedLine Citation:
PMID:  10562438     Owner:  NLM     Status:  MEDLINE    
A culture of cells was isolated from the organ of Corti of 2-week-old H-2Kb-tsA58 (Immortomouse) transgenic mice. All cells of these mice harbor a mutant of the simian virus 40 A-gene, encoding a thermolabile large T-antigen (Tag) protein. At 33 degrees C the Tag protein is functional and induces cell proliferation, but at 39 degrees C it is rapidly denatured and inactivated. Isolated organ of Corti cells growing at 33 degrees C were predominantly small, rounded or fusiform and proliferated rapidly. When moved to 39 degrees C, the cells reduced their rate of proliferation and differentiated into specific morphological phenotypes. Four cell lines were cloned by limiting dilution and characterized by immunofluorescence microscopy and Western blot. The cell lines, named OC-k1, OC-k2, OC-k3 and OC-k4, have been passaged at least 50 times with retention of a stable phenotype. These cell lines were all positive for the neuroepithelial precursor cell marker nestin and for the inner ear cell marker OCP2. In addition, the cells showed reactivity to epithelial and neuronal cell markers, but with a pattern of protein expression different for each clone and different between cells of the same clone growing at 33 degrees C or 39 degrees C. Some of the clones exhibited asymmetric cell division which is a characteristic commonly ascribed to stem cells. These cell lines can be used advantageously to study mechanisms and signals involved in the control of cell differentiation and morphogenesis of the mammalian inner ear and to isolate inner ear specific proteins.
F Kalinec; G Kalinec; M Boukhvalova; B Kachar
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell biology international     Volume:  23     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  1999  
Date Detail:
Created Date:  2000-04-04     Completed Date:  2000-04-04     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  175-84     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Section on Structural Cell Biology, Laboratory of Cellular Biology, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20850, USA.
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MeSH Terms
Antigens, Viral, Tumor / genetics
Cell Line, Transformed*
Cell Transformation, Viral*
Genes, Viral
Mice, Transgenic
Organ of Corti / pathology*
Simian virus 40*
Reg. No./Substance:
0/Antigens, Viral, Tumor

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