Document Detail

Establishment and characterization of adenoviral E1A immortalized cell lines derived from the rat suprachiasmatic nucleus.
MedLine Citation:
PMID:  10213449     Owner:  NLM     Status:  MEDLINE    
Primary cultured cells from the presumptive anlage of the rat suprachiasmatic nucleus (SCN) were immortalized by infection with a retroviral vector encoding the adenovirus 12S E1A gene. After drug selection, the resulting neural cell lines (SCN1.4 and SCN2.2) displayed (a) extended growth potential without evidence of transformed or tumorigenic properties, (b) expression of E1A protein within all cell nuclei, and (c) heterogeneous cell types in various stages of differentiation. A large proportion of the SCN1.4 and SCN2.2 cells were characterized by gliallike morphologies, but showed limited expression of corresponding cell type-specific antigens. In addition, both lines exhibited a stable population of cells with neuronlike characteristics. When treated so as to enhance differentiation, these cells were often distinguished by fine, long processes and immunocytochemical expression of neuronal markers and peptides found within SCN neurons in situ. Observations on SCN neuropeptide immunostaining, content, release, and mRNA expression followed a concordant pattern in which somatostatin and vasopressin cells were the most and least common peptidergic phenotypes in both lines, respectively. Since these results indicate that constituents of E1A-immortalized lines derived from the primordial SCN can differentiate into cells with phenotypes resembling parental peptidergic neurons, it will be critical to explore next whether these lines also retain the distinctive function of the SCN to generate circadian rhythms. Cloning of immortalized cell types could subsequently yield useful tools for studying the development of SCN glial and peptidergic cell types and delineating their distinct roles in mammalian circadian time-keeping.
D J Earnest; F Q Liang; S DiGiorgio; M Gallagher; B Harvey; B Earnest; G Seigel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of neurobiology     Volume:  39     ISSN:  0022-3034     ISO Abbreviation:  J. Neurobiol.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-06-15     Completed Date:  1999-06-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0213640     Medline TA:  J Neurobiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-13     Citation Subset:  IM    
Department of Human Anatomy and Medical Neurobiology, Texas A&M University Health Science Center, College of Medicine, College Station 77843-1114, USA.
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MeSH Terms
Adenovirus E1A Proteins / biosynthesis,  genetics*
Arginine Vasopressin / biosynthesis
Cell Culture Techniques / methods
Cell Division
Cell Line, Transformed
Cell Nucleus / ultrastructure
Gastrin-Releasing Peptide / biosynthesis
Neurons / cytology*,  metabolism,  virology
Neuropeptides / analysis,  biosynthesis*
RNA, Messenger / biosynthesis
Rats, Long-Evans
Somatostatin / biosynthesis
Suprachiasmatic Nucleus / cytology*
Transcription, Genetic
Vasoactive Intestinal Peptide / biosynthesis
Reg. No./Substance:
0/Adenovirus E1A Proteins; 0/Neuropeptides; 0/RNA, Messenger; 113-79-1/Arginine Vasopressin; 37221-79-7/Vasoactive Intestinal Peptide; 51110-01-1/Somatostatin; 80043-53-4/Gastrin-Releasing Peptide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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