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Establishment and characterization of an HPV16 E6/E7-expressing oral squamous cell carcinoma cell line with enhanced tumorigenicity.
MedLine Citation:
PMID:  20464641     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Oral squamous cell carcinoma (OSCC) is the most common malignant tumor in the oral and maxillofacial region. The mechanism of carcinogenesis of OSCC is still unclear. Based on the previous cell line, HIOEC-B(a)P-96 (HB96), which we obtained by HPV16 E6/E7-immortalized human oral epithelial cells (HIOEC) and benzo(a)pyrene inducement, we prepared a new HB-second generation cancer cell line (HB-2) by continuous passage. Its characteristics such as morphology, proliferation activity, karyotype, and tumorigenesis were studied. The HB-2 cells displayed uncontrolled cell division and lost contact inhibition leading to cell overlap. Cells were polygonal and unevenly shaped, with an increased nucleus versus plasma ratio. Increased proliferative activity was confirmed by MTT assays. The tumorigenicity was confirmed by tumor growth experiments in nude mice. Therefore, the HB-2 and HB96 cell lines are useful tools to study the mechanism of carcinogenesis of OSCC in vitro for future genomic and proteomic analyses.
Authors:
Dongxia Ye; Xiaojian Zhou; Hongya Pan; Qian Jiang; Laiping Zhong; Wantao Chen; Zhiyuan Zhang
Publication Detail:
Type:  Journal Article     Date:  2010-05-13
Journal Detail:
Title:  Medical oncology (Northwood, London, England)     Volume:  28     ISSN:  1559-131X     ISO Abbreviation:  Med. Oncol.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9435512     Medline TA:  Med Oncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1331-7     Citation Subset:  IM    
Affiliation:
Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, 639 Zhizao Ju Road, 200011, Shanghai, China.
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