Document Detail


Establishment and characterization of HKESC-1, a new cancer cell line from human esophageal squamous cell carcinoma.
MedLine Citation:
PMID:  10748291     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The establishment of an esophageal cancer cell line can facilitate the search for molecular mechanisms involved in esophageal carcinogenesis. A new human cancer cell line, HKESC-1, was established from a primary moderately-differentiated squamous cell carcinoma of the esophagus from a 47-year-old Hong Kong Chinese man. The pathological characteristics (morphology, immunohistochemical, and electron microscopic studies), the tumorigenecity in nude mice, the cytogenetic features, the DNA ploidy, and telomerase activity of the cell line were investigated. The HKESC-1 cells have been maintained continuously in vitro for more than 16 months and passaged over 96 times. HKESC-1 cells grow as a monolayer, with a doubling time of 46 hours. The HKESC-1 cells are of a squamous epithelial origin, as shown by their immunopositivity with the anti-cytokeratin antibodies and ultrastructural demonstration of tonofilaments and desmosomes. The HKESC-1 cells possess characteristics of malignancy because they are highly tumorigenic in nude mice and have strong telomerase activity. The HKESC-1 cells had an aneuploid DNA content, as demonstrated by flow cytometric analysis. Cytogenetic analysis revealed hyperdiploidy of greater than 50 in 80% of analyzable metaphases. Chromosome gains and losses were common, and loss of the Y chromosome was a consistent numerical aberration. Additionally, many structural chromosomal abnormalities were encountered, with frequent breakpoints at 1p32, 7p22, 7q34, and 20q13. This newly established cell line serves as a useful model for studying the molecular pathogenesis, and testing new therapeutic reagents for esophageal squamous cell carcinoma.
Authors:
Y Hu; K Y Lam; T S Wan; W Fang; E S Ma; L C Chan; G Srivastava
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer genetics and cytogenetics     Volume:  118     ISSN:  0165-4608     ISO Abbreviation:  Cancer Genet. Cytogenet.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-05-02     Completed Date:  2000-05-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7909240     Medline TA:  Cancer Genet Cytogenet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  112-20     Citation Subset:  IM    
Affiliation:
Department of Pathology, The University of Hong Kong, Hong Kong, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Squamous Cell / genetics,  pathology*
Cell Line*
Chromosome Aberrations
DNA, Neoplasm / analysis
Esophageal Neoplasms / genetics,  pathology*
Flow Cytometry
Humans
Karyotyping
Male
Mice
Mice, Nude
Middle Aged
Neoplasm Transplantation
Telomerase / analysis
Transplantation, Heterologous
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; EC 2.7.7.49/Telomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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