| Establishment and characterization of 12S adenoviral E1A immortalized rat submandibular gland epithelial cells. | |
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MedLine Citation:
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PMID: 2164809 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rat submandibular gland (RSMG) cells have been immortalized with a retrovirus vector which encodes the adenovirus 12S E1A gene product. The immortalized cells were epithelial-like in nature and displayed a beta-adrenergic coupled rise in intracellular cAMP upon exposure to norepinephrine. Western- and lectin-blotting experiments of cell lysates demonstrated the presence of blood group A-reactive oligosaccharides. Such oligosaccharides are characteristic of RSMG mucin-glycoproteins. These cells appear to be suitable for use in analysis of cell-specific factors which regulate RSMG glycosylation. |
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Authors:
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X J He; D P Frank; L A Tabak |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 170 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1990 Jul |
Date Detail:
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Created Date: 1990-08-21 Completed Date: 1990-08-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 336-43 Citation Subset: IM |
Affiliation:
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Department of Dental Research, School of Medicine and Dentistry, University of Rochester, NY 14642. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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ABO Blood-Group System
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immunology Adenovirus Early Proteins Animals Cell Division / drug effects Cell Line Cloning, Molecular Cyclic AMP / metabolism Epithelial Cells Epithelium / drug effects, metabolism Norepinephrine / pharmacology Oligosaccharides / immunology, metabolism Oncogene Proteins, Viral / pharmacology* Rats Submandibular Gland / cytology*, drug effects, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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DE 08108/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/ABO Blood-Group System; 0/Adenovirus Early Proteins; 0/Oligosaccharides; 0/Oncogene Proteins, Viral; 51-41-2/Norepinephrine; 60-92-4/Cyclic AMP |
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