Document Detail


Establishment and characteristics of an animal model for isolated pancreatic trauma.
MedLine Citation:
PMID:  23007015     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: There is not any stable, repeatable, and clinically simulated animal model of isolated pancreatic trauma been established as yet. The aim of our study is to establish a small animal model that mimics the pathophysiology of isolated pancreatic blast trauma and to study the features of traumatic condition in the model.
METHODS: Rats were divided into three groups: calibrate group, impact group, and control group. The pancreas in the impact group was injured by BIM-III model of the biotical impact machine with the impact pressures of 800 kPa, 600 kPa, 400 kPa, 200 kPa, and 100 kPa. The mortality, intra-abdominal structural changes, amylase (AMS) and lipase (LPS) activities of serum, and pathologic alterations of the pancreas were examined at 24 hours after trauma. We especially observed these changes and the variation of proliferation index in the rats impacted with 400 kPa pressures after 6 hours, 24 hours, 72 hours, and 7 days.
RESULTS: Pancreatic edema, hemorrhage, cell necrosis, and vacuolization were found accompanied with marked increase in serum AMS and LPS. Severity of pancreatic histologic injury was associated with the impact pressure level and varied over time in the 400-kPa group. Applying 400 kPa impact pressure could be able to provide ideal research samples. Pancreatic trauma also induced compensatory proliferation of pancreatic cells.
CONCLUSION: A practical animal model of isolated pancreatic blunt trauma could be established under 400 kPa pressures. It is ideal for further pathologic and therapeutic study. The combined determination of serum AMS and LPS, particularly the dynamic variation of these enzymes, might assist the diagnosis and prognosis of pancreatic trauma. Further research should focus on the promotion of the cellular regenerative response.
Authors:
Ruiwu Dai; Guangyu Chen; Zhu Huang; Hongtao Yan; Ning Lin; Yong Yan; Tao Wang; Lijun Tang
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of trauma and acute care surgery     Volume:  73     ISSN:  2163-0763     ISO Abbreviation:  J Trauma Acute Care Surg     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-25     Completed Date:  2012-11-20     Revised Date:  2013-09-25    
Medline Journal Info:
Nlm Unique ID:  101570622     Medline TA:  J Trauma Acute Care Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  648-53     Citation Subset:  AIM; IM    
Affiliation:
General Surgery Center, General Hospital of the Chinese People's Liberation Army, Chengdu Military Region, Chengdu, China.
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MeSH Terms
Descriptor/Qualifier:
Abdominal Injuries / mortality,  pathology*
Analysis of Variance
Animals
Biopsy, Needle
Disease Models, Animal*
Immunohistochemistry
Kaplan-Meier Estimate
Male
Pancreas / injuries*,  pathology*
Pancreatic Function Tests
Random Allocation
Rats
Rats, Wistar
Risk Factors
Sensitivity and Specificity
Statistics, Nonparametric
Survival Rate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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