Document Detail

Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP.
MedLine Citation:
PMID:  18533106     Owner:  NLM     Status:  MEDLINE    
Microprocessor, the complex of Drosha and DGCR8, promotes the processing of primary microRNA to precursor microRNA, which is a crucial step for microRNA maturation. So far, no convenient assay systems have been developed for observing this step in vivo. Here we report the establishment of highly sensitive cellular systems where we can visually monitor the function of Microprocessor. During a series of screening of transfectants with fusion genes of the EGFP cDNA and primary microRNA genes, we have obtained certain cell lines where introduction of siRNA against DGCR8 or Drosha strikingly augments GFP signals. In contrast, these cells have not responded to Dicer siRNA; thus they have a unique character that GFP signals should be negatively and specifically correlated to the action of Microprocessor among biogenesis of microRNA. These cell lines can be useful tools for real-time analysis of Microprocessor action in vivo and identifying its novel modulators.
Motomu Tsutsui; Hitoki Hasegawa; Koichi Adachi; Maiko Miyata; Peng Huang; Naoki Ishiguro; Michinari Hamaguchi; Takashi Iwamoto
Related Documents :
18050486 - Cell fusion.
14745236 - Forming a multinucleated cell: molecules that regulate myoblast fusion.
19572016 - Acquisition of cell-cell fusion activity by amino acid substitutions in spike protein d...
2183466 - Hemadsorption and fusion inhibition activities of hemagglutinin analyzed by vaccinia vi...
8174636 - Alterations in the localization of f-actin, fibronectin, and thrombospondin occur prior...
22689816 - Role of lipid rafts and flagellin in invasion of colonic epithelial cells by shiga-toxi...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-03
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  372     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-06-25     Completed Date:  2008-07-24     Revised Date:  2011-11-03    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  856-61     Citation Subset:  IM    
Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Sowa-ku, Nagoya, Aichi 466-8550, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Base Sequence
DEAD-box RNA Helicases / genetics
Endoribonucleases / genetics
Gene Fusion
Green Fluorescent Proteins / analysis,  genetics
MicroRNAs / genetics,  metabolism*
Molecular Sequence Data
NIH 3T3 Cells*
Proteins / genetics,  metabolism*
Ribonuclease III / genetics,  metabolism*
Reg. No./Substance:
0/DGCR8 protein, human; 0/MicroRNAs; 0/Proteins; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins; EC 3.1.-/Endoribonucleases; EC protein, human; EC protein, human; EC III; EC 3.6.1.-/DEAD-box RNA Helicases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Multiple roles for nuclear localization signal (NLS, aa 442-472) of receptor interacting protein 3 (...
Next Document:  Tropoelastin regulates chemokine expression in fibroblasts in Costello syndrome.