Document Detail


Establishment of cell lines from somatic cell hybrids between human monocytes and mouse myeloma cells.
MedLine Citation:
PMID:  6606677     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To study human monocyte functions, we attempted to immortalize human monocytes by producing somatic cell hybrids between such monocytes and the mouse myeloma cell line NSI. In this study we report the successful establishment of eight hybrid cell lines that have been grown in culture for more than a year, and some of them retained part of the human chromosome complement, as well as monocyte markers and activities. Karyotype analysis of these hybrid lines revealed that cells of seven out of eight of the lines contained one to 16 human chromosomes and in four of them, more than nine human chromosomes were observed. Several of the cell lines expressed monocytic markers and functions. Thus, in two of the hybrid lines nonspecific esterase could be demonstrated in 10 to 29% of the cells, and Fc receptors were demonstrated in three of the hybrid cell lines. Significant levels of human ferritin were detected in one of the lines, and two other cell lines secreted interleukin 1-like substance into the culture medium. These results encourage us to use human-mouse somatic cell hybridization as an approach for the establishment of human monocyte cell lines, which will preserve their functions and produce monocyte-derived factors.
Authors:
A J Treves; Z Fuks; R Voss; T Tal; V Barak; A M Konijn; R Kaplan; R Laskov
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  132     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1984 Feb 
Date Detail:
Created Date:  1984-02-23     Completed Date:  1984-02-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  690-4     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fusion
Cell Line
Chromosomes, Human
Ferritins / analysis
Humans
Hybrid Cells / immunology*
Interleukin-1 / physiology
Karyotyping
Mice
Monocytes / enzymology,  immunology*,  metabolism
Plasmacytoma / immunology*
Receptors, Fc / analysis
Chemical
Reg. No./Substance:
0/Interleukin-1; 0/Receptors, Fc; 9007-73-2/Ferritins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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