Document Detail

Establishment of a blood purification system for renal failure rats using small-size dialyzer membranes.
MedLine Citation:
PMID:  23190517     Owner:  NLM     Status:  In-Data-Review    
Hemodialysis techniques for small animals have not been established because no small dialysis apparatus has been available. We recently developed a small-size dialyzer and established an appropriate blood purification system for small animals. To confirm the appropriate dialysate flow rate, bovine blood was dialyzed for 60 min at a fixed blood flow rate of 1.0 mL/min and variable dialysate flow rates. Blood urea nitrogen and creatinine levels decreased significantly at a dialysate flow rate of 5 mL/min (from 13.7 ± 0.2 to 10.3 ± 1.2 mg/dL and 1.07 ± 0.15 to 0.61 ± 0.12 mg/dL, respectively, P < 0.05). To determine the appropriate in vivo conditions, extracorporeal circulation was performed in anesthetized male Sprague-Dawley rats at a dialysate flow rate of 0.0 mL/min, for 240 min, and at variable blood flow rates. Extracorporeal circulation was successful at a blood flow rate of 1.0 mL/min, but not 1.5 mL/min. To establish in vivo hemodialysis conditions, we used the animal model of end stage renal failure. Sprague-Dawley rats were fed a 0.75% adenine-containing diet for 3 weeks, after which they received hemodialysis for 120 min at a dialysate and blood flow rate of 5.0 and 1.0 mL/min, respectively. There were no significant changes in systolic blood pressure or heart rate during dialysis. Thus, this blood purification system can be safely used for small animals at a dialysate flow rate of 5.0 mL/min and a blood flow rate of 1.0 mL/min. This system provides a basis for further research on hemodialysis therapy.
Daisuke Yorimitsu; Minoru Satoh; Masahide Koremoto; Yoshisuke Haruna; Hajime Nagasu; Atsunori Kuwabara; Tamaki Sasaki; Naoki Kashihara
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Publication Detail:
Type:  Journal Article     Date:  2012-08-07
Journal Detail:
Title:  Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy     Volume:  16     ISSN:  1744-9987     ISO Abbreviation:  Ther Apher Dial     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101181252     Medline TA:  Ther Apher Dial     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  566-72     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Therapeutic Apheresis and Dialysis © 2012 International Society for Apheresis.
Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama Asahi Kasei Kuraray Medical Co, Ltd, Chiyodaku, Tokyo, Japan.
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