| Establishment of a blood purification system for renal failure rats using small-size dialyzer membranes. | |
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MedLine Citation:
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PMID: 23190517 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Hemodialysis techniques for small animals have not been established because no small dialysis apparatus has been available. We recently developed a small-size dialyzer and established an appropriate blood purification system for small animals. To confirm the appropriate dialysate flow rate, bovine blood was dialyzed for 60 min at a fixed blood flow rate of 1.0 mL/min and variable dialysate flow rates. Blood urea nitrogen and creatinine levels decreased significantly at a dialysate flow rate of 5 mL/min (from 13.7 ± 0.2 to 10.3 ± 1.2 mg/dL and 1.07 ± 0.15 to 0.61 ± 0.12 mg/dL, respectively, P < 0.05). To determine the appropriate in vivo conditions, extracorporeal circulation was performed in anesthetized male Sprague-Dawley rats at a dialysate flow rate of 0.0 mL/min, for 240 min, and at variable blood flow rates. Extracorporeal circulation was successful at a blood flow rate of 1.0 mL/min, but not 1.5 mL/min. To establish in vivo hemodialysis conditions, we used the animal model of end stage renal failure. Sprague-Dawley rats were fed a 0.75% adenine-containing diet for 3 weeks, after which they received hemodialysis for 120 min at a dialysate and blood flow rate of 5.0 and 1.0 mL/min, respectively. There were no significant changes in systolic blood pressure or heart rate during dialysis. Thus, this blood purification system can be safely used for small animals at a dialysate flow rate of 5.0 mL/min and a blood flow rate of 1.0 mL/min. This system provides a basis for further research on hemodialysis therapy. |
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Authors:
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Daisuke Yorimitsu; Minoru Satoh; Masahide Koremoto; Yoshisuke Haruna; Hajime Nagasu; Atsunori Kuwabara; Tamaki Sasaki; Naoki Kashihara |
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Publication Detail:
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Type: Journal Article Date: 2012-08-07 |
Journal Detail:
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Title: Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy Volume: 16 ISSN: 1744-9987 ISO Abbreviation: Ther Apher Dial Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-11-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101181252 Medline TA: Ther Apher Dial Country: Australia |
Other Details:
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Languages: eng Pagination: 566-72 Citation Subset: IM |
Copyright Information:
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© 2012 The Authors. Therapeutic Apheresis and Dialysis © 2012 International Society for Apheresis. |
Affiliation:
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Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama Asahi Kasei Kuraray Medical Co, Ltd, Chiyodaku, Tokyo, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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