| Establishment and biological characteristics of oxaliplatin-resistant human colon cancer cell lines. | |
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MedLine Citation:
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PMID: 20591218 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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BACKGROUND AND OBJECTIVE: Chemotherapy is the main treatment for colon cancer, while multidrug-resistance is the main reason for chemotherapy failure and tumor relapse. This study was to establish two oxaliplatin-resistant colon cancer cell lines and evaluate their biological characteristics. METHODS: Oxaliplatin-resistant colon cancer cell lines SW620/L-OHP and lovo/L-OHP were established in vitro by continuous exposure to oxaliplatin (L-OHP) of low and gradually increased concentration. Growth curve, cross-resistance and resistance index of the oxaliplatin-resistant cell lines to various anti-cancer agents were determined by CCK8 assay. The expressions of P-glycoprotein (P-gp), multidrug-resistance protein 1 (MRP1) and MRP2 were detected by Western blot. Cell cycle distribution as well as the expression of CD133 and CD44 were measured by flow cytometry. RESULTS: It took 10 months to establish the SW620/L-OHP and LoVo/L-OHP cell lines with stable resistance to oxaliplatin. Cross-resistance to 5-fluorouracil, etoposide, cisplatin, vincristine and epirubicin but not to paclitaxel was observed. Longer doubling time, higher proportion of cells in G(0)/G(1) phase and lower proportion in G(2)/M phase were observed in the two oxaliplatin-resistant cell lines compared with their parental cell lines. The expression of MRP2 in the oxaliplatin-resistant cells was up-regulated, while those of P-gp and MRP1 had no significant change. CD133 was overexpressed while CD44 level remained unchanged in SW620/L-OHP and LoVo/L-OHP cells. CONCLUSIONS: SW620/L-OHP and LoVo/L-OHP cell lines show a typical and stably resistant phenotype and may be used as research models. |
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Authors:
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Zhen Liu; Meng Qiu; Qiu-Lin Tang; Ming Liu; Nan Lang; Feng Bi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Chinese journal of cancer Volume: 29 ISSN: 1944-446X ISO Abbreviation: Chin J Cancer Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101498232 Medline TA: Chin J Cancer Country: China |
Other Details:
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Languages: eng Pagination: 661-7 Citation Subset: IM |
Affiliation:
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Department of Medical Oncology ,West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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