Document Detail


Establishment and biological characteristics of human multiple myeloma cell line CZ-1.
MedLine Citation:
PMID:  14733786     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: There were only 3 multiple myeloma (MM) cell lines established in China. In this study, we succeeded in establishing a novel MM cell line and analyzed its biological characteristics. METHODS: Mononuclear cells isolated from the peripheral blood (PB) and bone marrow (BM) of a patient with advanced MM (lambda light chain type) were cultured in medium. Cell morphology was analyzed by Wright-Giemsa-staining and cytochemical staining, immunophenotyping by flow cytometry and cytogenetic analysis by chromosome RHG-banding technique. Quantitative fluorescent polymerase chain reaction (PCR) was used to detect Epstein - Barr virus (EBV) DNA. RESULTS: The established cell line could survive and proliferate in the presence of feeder cells or conditioned medium. The cells secreted lambda light chain and were negative for EBV. The Wright-Giemsa-staining showed typical plasmablast or plasma cell morphology. The cytochemical staining of the cells showed the following reactivity patterns: positive for acid phosphatase, negative for myeloperoxidase. The immunoprofile of the cells was concordant with that of MM cells: positive for CD10, CD28, CD38, CD138, CD56, CD49d, CD44, CD54 and CD58, negative for CD19, CD40, CD95, CD95L, CD34, CD2 and CD5. The cytogenetic analysis showed complex chromosome abnormality of i (1q+), 8q+, 13q+, i (17q), i (18q) and +M. There was no difference in morphology, immunophenotype and cytogenetics between cells from PB and BM. CONCLUSIONS: An MM cell line secreting lambda light chain named CZ-1 was established. The cells from both PB and BM have the same biological characteristics.
Authors:
Jian Hou; Fa-ying Lin; Bo Zhang; Ling-zhen Zhang; Si-qi Ding
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Chinese medical journal     Volume:  117     ISSN:  0366-6999     ISO Abbreviation:  Chin. Med. J.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-01-21     Completed Date:  2004-05-03     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7513795     Medline TA:  Chin Med J (Engl)     Country:  China    
Other Details:
Languages:  eng     Pagination:  115-9     Citation Subset:  IM    
Affiliation:
Department of Hematology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. houjian167@sohu.com
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Chromosome Aberrations
Herpesvirus 4, Human / isolation & purification
Humans
Male
Middle Aged
Multiple Myeloma / genetics*,  pathology*
Polymerase Chain Reaction

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