Document Detail

Establishment of an acute graft-versus-host disease model following liver transplantation in donor-dominant one-way major histocompatibility complex matching rats.
MedLine Citation:
PMID:  19545756     Owner:  NLM     Status:  MEDLINE    
Acute graft-versus-host disease is an uncommon but devastating complication following liver transplantation (LTx-aGVHD). We investigated whether a rat model of LTx-aGVHD could be established using Lewis rat donors and (LewisXBN)F1 rats as recipients, which provides favorable conditions for studies of graft-versus-host reaction or disease. Replacement of (LewisXBN)F1 livers by Lewis livers alone was not sufficient to induce aGVHD; all recipients grew in a normal pattern as the syngeneic liver transplantation (LT) from Lewis to Lewis rat. However, when various numbers of donor splenocytes (1 x 10(8), 2 x 10(8), 3 x 10(8), 4 x 10(8)) were transferred simultaneously with the LT, the morbidities of lethal aGVHD were 16.7%, 50%, 83.3%, and 100%, respectively. The clinical courses as well as histologic analyses of skin and colon showed typical aGVHD characteristics. However, unlike transfusion-associated aGVHD, the liver graft was not involved. These clinical and histologic characteristics of aGVHD were consistent with those in humans who develop aGVHD after LT. Thus, a reproducible rat model of LTx-aGVHD was developed by performing LT from Lewis to (LewisXBN)F1 rats in combination with donor splenocyte transfusion. This model may be useful for further studies on the mechanisms and effective treatment modalities for LTx-aGVHD.
F Xue; W Chen; X G Wang; L Liang; X L Bai; L Y Wang; H P Wang; T B Liang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transplantation proceedings     Volume:  41     ISSN:  1873-2623     ISO Abbreviation:  Transplant. Proc.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-23     Completed Date:  2009-09-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0243532     Medline TA:  Transplant Proc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1914-20     Citation Subset:  IM    
Department of Hepatobiliary and Pancreatic Surgery, Key Laboratory of Combined Multi-organ Transplantation of Ministry of Public Health, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
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MeSH Terms
Adoptive Transfer / methods
Graft vs Host Disease / immunology*
Histocompatibility Testing / methods*
Liver Transplantation / immunology*
Major Histocompatibility Complex*
Models, Immunological
Rats, Inbred BN
Rats, Inbred Lew
Spleen / transplantation*
Transplantation, Homologous

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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