| Establishment of Intestinal Epithelial Cell Lines from Adult Mouse Small and Large Intestinal Crypts. | |
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MedLine Citation:
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PMID: 21597185 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Small and large intestinal epithelial cell (IEC) lines were established from adult murine intestinal crypts. Both established small and large IECs line (named aMoS7 and aMoC1 respectively) expressed epithelial markers. Similarly to IECs isolated from adult mouse intestines, the expression of major histocompatibility complex (MHC) class II molecules was induced by interferon-γ-treatment in both established cell lines. This expression of MHC class II molecules was higher in small intestinal aMoS7 cells than in large intestinal aMoC1 cells. Treatment with lipopolysaccharide and with ligands of Toll-like receptors 1, 2, 3, and 7 induced secretion of interleukin-6 from both adult IEC lines. These results suggest that the aMoS7 and aMoC1 cell lines can serve as useful tools in analyzing the immunological functions of IECs, especially in studying the IEC response to microbial components and its antigen presenting ability. |
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Authors:
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Taku Iwamoto; Kiyoshi Yamada; Makoto Shimizu; Mamoru Totsuka |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-20 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: - ISSN: 1347-6947 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Applied Biological Chemistry, The University of Tokyo. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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