| Establishment of an efficient fermentation system of gamma-aminobutyric acid by a lactic acid bacterium, Enterococcus avium G-15, isolated from carrot leaves. | |
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MedLine Citation:
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PMID: 20930374 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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In the present study, we successfully isolated a carrot leaf-derived lactic acid bacterium that produces gamma-aminobutyric acid (GABA) from monosodium L-glutamate (L-MSG) at a hyper conversion rate. The GABA-producing bacterium, identified as Enterococcus (E.) avium G-15, produced 115.7±6.4 g/l GABA at a conversion rate of 86.0±5.0% from the added L-MSG under the optimum culture condition by a continuous L-MSG feeding method using a jar-fermentor, suggesting that the bacterium displays a great potential ability for the commercial-level fermentation production of GABA. Using the reverse transcription polymerase chain reaction (RT-PCR) method, we analyzed the expression of genes for the GABA transporter and glutamate decarboxylase, designated gadT and gadG, respectively, which were cloned from the E. avium G-15 chromosome. Both genes were expressed even without the added L-MSG, but their expression was enhanced by the addition of L-MSG. |
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Authors:
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Takayoshi Tamura; Masafumi Noda; Moeko Ozaki; Masafumi Maruyama; Yasuyuki Matoba; Takanori Kumagai; Masanori Sugiyama |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biological & pharmaceutical bulletin Volume: 33 ISSN: 1347-5215 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-10-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
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Languages: eng Pagination: 1673-9 Citation Subset: IM |
Affiliation:
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Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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