Document Detail

Establishment of an ELISA for measuring bovine pregnancy-associated glycoprotein in serum or milk and its application for early pregnancy detection.
MedLine Citation:
PMID:  19032429     Owner:  NLM     Status:  MEDLINE    
It has been shown in several mammalian species that during pregnancy, trophoblast cells express a range of pregnancy-associated glycoproteins (PAG). The presence of PAG in the maternal serum of cows may serve as an indicator of pregnancy from day 28 after AI onward. The present study addresses (1) conversion of an existing PAG-RIA to a competitive double antibody ELISA using a polyclonal anti-bPAG-IgG and an anti-rabbit-IgG raised in sheep for coating and (2) application of newly established ELISA to test its suitability for pregnancy detection by measuring PAG in serum or milk. The intraassay coefficients of variation (CV) for the PAG-ELISA were 2-14% for serum and 10-12% for milk; the corresponding interassay CVs were 8-22% and 12-22%, respectively. Pregnancy-associated glycoprotein profiles established in milk and serum of 12 pregnant cows showed a characteristic pattern with measurable amounts from approximately day 20 onwards in serum and from day 60 onwards in milk. In a field trial, serum PAG was determined in 397 cows sampled between 20 and 50 days after insemination. The outcome was that, pregnancy could reliably be diagnosed from day 28 onwards in serum and from day 150 onwards in milk. In conclusion, it may be stated that the established ELISA provides an efficient and reliable means of pregnancy diagnosis that will, in our judgement, gain in popularity with cattle breeding. The ELISA proved to be an adequate and efficient way of measuring PAG in maternal serum or milk and will be a useful means of pregnancy detection in cows.
M Friedrich; W Holtz
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2008-11-20
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  45     ISSN:  1439-0531     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-08     Completed Date:  2010-04-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  142-6     Citation Subset:  IM    
Institute of Animal Breeding and Genetics, G?ttingen, Germany.
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MeSH Terms
Breeding / methods
Enzyme-Linked Immunosorbent Assay / methods,  veterinary*
Gestational Age
Glycoproteins / analysis*,  blood
Immunoglobulin G / immunology
Insemination, Artificial / veterinary
Milk / chemistry*
Pregnancy Proteins / analysis*,  blood
Pregnancy Tests / methods,  veterinary*
Rabbits / immunology
Sensitivity and Specificity
Time Factors
Reg. No./Substance:
0/Glycoproteins; 0/Immunoglobulin G; 0/Pregnancy Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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