Document Detail

Establishment and characterization of renal carcinoma cell lines from a Bhd gene mutant (Nihon) rat.
MedLine Citation:
PMID:  19816092     Owner:  NLM     Status:  MEDLINE    
A germline insertion of a single nucleotide in the rat homologue of the human Birt-Hogg-Dubé (BHD) gene gives rise to dominantly inherited renal cell carcinoma (RCC) in the Nihon rat model. In this study, we established 7 lines (NR cell lines NR22, 24, 32, 45, 49, 54 and 64) from an RCC found in a Nihon rat. All cell lines consisted mainly of round or polygonal cells arranged in a cobblestone-like growth pattern. Cells of NR cell lines had abundant cytoplasm and tight junctions as well as microvilli on electron microscopy and were positive for cytokeratin on immunocytochemistry. Cell lines NR22, 24 and 32 showed rapid growth, whereas the growth of the remaining lines was very slow. While the modal chromosome number of lines NR24, 45 and 54 was 42, the remaining lines exhibited aberrant modal numbers ranging from 70 to 96. All NR cell lines formed tumors at subcutaneous inoculation sites in nude mice, and tumors from lines NR54 and 64 developed pulmonary metastases. All NR cell lines had a germline mutation in the rat Bhd gene in the gene analysis. NR cell lines would prove valuable experimental tools for studies on unique functions of the Bhd gene and renal carcinogenesis.
Izumi Matsumoto; Mami Kouchi; Kazuo Okimoto; Kazuyasu Kijima; Tadayoshi Ueda; Youko Hirayama; Tadashi Inoue; Takaki Seki; Okio Hino
Publication Detail:
Type:  Journal Article     Date:  2009-10-07
Journal Detail:
Title:  Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine     Volume:  30     ISSN:  1423-0380     ISO Abbreviation:  Tumour Biol.     Publication Date:  2009  
Date Detail:
Created Date:  2010-01-06     Completed Date:  2010-02-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8409922     Medline TA:  Tumour Biol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  249-56     Citation Subset:  IM    
Copyright Information:
(c) 2009 S. Karger AG, Basel.
Safety Research Laboratories, Dainippon Sumitomo Pharma Co., Ltd., Osaka, Japan.
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MeSH Terms
Base Sequence
Carcinoma, Renal Cell / genetics,  metabolism,  pathology*
Cell Line, Tumor
Cell Proliferation
DNA Mutational Analysis
Keratins / analysis
Liver Neoplasms / genetics,  metabolism,  pathology*
Loss of Heterozygosity
Mice, Inbred BALB C
Mice, Nude
Microscopy, Electron
Microvilli / ultrastructure
Neoplasms, Experimental / genetics,  metabolism,  pathology
Proto-Oncogene Proteins / genetics*
Rats, Mutant Strains
Tight Junctions / ultrastructure
Transplantation, Heterologous
Tumor Suppressor Proteins / genetics*
Vimentin / analysis
Reg. No./Substance:
0/Bhd protein, mouse; 0/Proto-Oncogene Proteins; 0/Tumor Suppressor Proteins; 0/Vimentin; 68238-35-7/Keratins

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