| Establishment and characterization of renal carcinoma cell lines from a Bhd gene mutant (Nihon) rat. | |
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MedLine Citation:
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PMID: 19816092 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A germline insertion of a single nucleotide in the rat homologue of the human Birt-Hogg-Dubé (BHD) gene gives rise to dominantly inherited renal cell carcinoma (RCC) in the Nihon rat model. In this study, we established 7 lines (NR cell lines NR22, 24, 32, 45, 49, 54 and 64) from an RCC found in a Nihon rat. All cell lines consisted mainly of round or polygonal cells arranged in a cobblestone-like growth pattern. Cells of NR cell lines had abundant cytoplasm and tight junctions as well as microvilli on electron microscopy and were positive for cytokeratin on immunocytochemistry. Cell lines NR22, 24 and 32 showed rapid growth, whereas the growth of the remaining lines was very slow. While the modal chromosome number of lines NR24, 45 and 54 was 42, the remaining lines exhibited aberrant modal numbers ranging from 70 to 96. All NR cell lines formed tumors at subcutaneous inoculation sites in nude mice, and tumors from lines NR54 and 64 developed pulmonary metastases. All NR cell lines had a germline mutation in the rat Bhd gene in the gene analysis. NR cell lines would prove valuable experimental tools for studies on unique functions of the Bhd gene and renal carcinogenesis. |
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Authors:
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Izumi Matsumoto; Mami Kouchi; Kazuo Okimoto; Kazuyasu Kijima; Tadayoshi Ueda; Youko Hirayama; Tadashi Inoue; Takaki Seki; Okio Hino |
Publication Detail:
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Type: Journal Article Date: 2009-10-07 |
Journal Detail:
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Title: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine Volume: 30 ISSN: 1423-0380 ISO Abbreviation: Tumour Biol. Publication Date: 2009 |
Date Detail:
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Created Date: 2010-01-06 Completed Date: 2010-02-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8409922 Medline TA: Tumour Biol Country: Switzerland |
Other Details:
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Languages: eng Pagination: 249-56 Citation Subset: IM |
Copyright Information:
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(c) 2009 S. Karger AG, Basel. |
Affiliation:
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Safety Research Laboratories, Dainippon Sumitomo Pharma Co., Ltd., Osaka, Japan. izumi-matsumoto@ds-pharma.co.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Carcinoma, Renal Cell / genetics, metabolism, pathology* Cell Line, Tumor Cell Proliferation DNA Mutational Analysis Female Immunohistochemistry Keratins / analysis Liver Neoplasms / genetics, metabolism, pathology* Loss of Heterozygosity Male Mice Mice, Inbred BALB C Mice, Nude Microscopy, Electron Microvilli / ultrastructure Mutation* Neoplasms, Experimental / genetics, metabolism, pathology Proto-Oncogene Proteins / genetics* Rats Rats, Mutant Strains Tight Junctions / ultrastructure Transplantation, Heterologous Tumor Suppressor Proteins / genetics* Vimentin / analysis |
| Chemical | |
Reg. No./Substance:
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0/Bhd protein, mouse; 0/Proto-Oncogene Proteins; 0/Tumor Suppressor Proteins; 0/Vimentin; 68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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