Document Detail


Establishment and characterization of immortalized human amniotic epithelial cells.
MedLine Citation:
PMID:  23298399     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human amniotic epithelial cells (HAEs) have a low immunogenic profile and possess potent immunosuppressive properties. HAEs also have several characteristics similar to stem cells, and they are discarded after parturition. Thus, they could potentially be used in cell therapy with fewer ethical problems. HAEs have a short life, so our aim is to establish and characterize immortalized human amniotic epithelial cells (iHAEs). HAEs were introduced with viral oncogenes E6/E7 and with human telomerase reverse transcriptase (hTERT) to create iHAEs. These iHAEs have proliferated around 200 population doublings (PDs) for at least 12 months. High expression of stem cell markers (Oct 3/4, Nanog, Sox2, Klf4) and epithelial markers (CK5, CK18) were detected by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). These iHAEs were expanded in ultra-low-attachment dishes to form spheroids similarly to epithelial stem/precursor cells. High expression of mesenchymal (CD44, CD73, CD90, CD105) and somatic (CD24, CD29, CD271, Nestin) stem cell markers was detected by flow cytometry. The iHAEs showed adipogenic, osteogenic, neuronal, and cardiac differentiation abilities. In conclusion, the immortalization of HAEs with the characteristics of stem cells has been established, allowing these iHAEs to become useful for cell therapy and regenerative medicine.
Authors:
Kaixuan Zhou; Chika Koike; Toshiko Yoshida; Motonori Okabe; Moustafa Fathy; Satoru Kyo; Tohru Kiyono; Shigeru Saito; Toshio Nikaido
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-08
Journal Detail:
Title:  Cellular reprogramming     Volume:  15     ISSN:  2152-4998     ISO Abbreviation:  Cell Reprogram     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-05     Completed Date:  2013-07-12     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  101528176     Medline TA:  Cell Reprogram     Country:  United States    
Other Details:
Languages:  eng     Pagination:  55-67     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amnion / cytology*,  metabolism*
Antigens, CD / biosynthesis,  genetics
Cell Differentiation / genetics
Cell Line, Transformed / cytology*,  metabolism*
Epithelial Cells / cytology*,  metabolism*
Humans
Papillomavirus E7 Proteins / biosynthesis,  genetics
Spheroids, Cellular / cytology,  metabolism
Telomerase / biosynthesis,  genetics
Transcription Factors / biosynthesis,  genetics
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Papillomavirus E7 Proteins; 0/Transcription Factors; EC 2.7.7.49/TERT protein, human; EC 2.7.7.49/Telomerase
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