| Establishment and Characterization of Immortalized Human Amniotic Epithelial Cells. | |
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MedLine Citation:
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PMID: 23298399 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Abstract Human amniotic epithelial cells (HAEs) have a low immunogenic profile and possess potent immunosuppressive properties. HAEs also have several characteristics similar to stem cells, and they are discarded after parturition. Thus, they could potentially be used in cell therapy with fewer ethical problems. HAEs have a short life, so our aim is to establish and characterize immortalized human amniotic epithelial cells (iHAEs). HAEs were introduced with viral oncogenes E6/E7 and with human telomerase reverse transcriptase (hTERT) to create iHAEs. These iHAEs have proliferated around 200 population doublings (PDs) for at least 12 months. High expression of stem cell markers (Oct 3/4, Nanog, Sox2, Klf4) and epithelial markers (CK5, CK18) were detected by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). These iHAEs were expanded in ultra-low-attachment dishes to form spheroids similarly to epithelial stem/precursor cells. High expression of mesenchymal (CD44, CD73, CD90, CD105) and somatic (CD24, CD29, CD271, Nestin) stem cell markers was detected by flow cytometry. The iHAEs showed adipogenic, osteogenic, neuronal, and cardiac differentiation abilities. In conclusion, the immortalization of HAEs with the characteristics of stem cells has been established, allowing these iHAEs to become useful for cell therapy and regenerative medicine. |
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Authors:
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Kaixuan Zhou; Chika Koike; Toshiko Yoshida; Motonori Okabe; Moustafa Fathy; Satoru Kyo; Tohru Kiyono; Shigeru Saito; Toshio Nikaido |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-8 |
Journal Detail:
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Title: Cellular reprogramming Volume: - ISSN: 2152-4998 ISO Abbreviation: Cell Reprogram Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101528176 Medline TA: Cell Reprogram Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1 Department of Regenerative Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama , Toyama, Japan . |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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