| Establishing equivalence for microbial growth inhibitory effects ("iso-hurdle rules") by analysing disparate data on Listeria monocytogenes with a gamma-type predictive model. | |
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MedLine Citation:
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PMID: 22156426 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Preservative factors act as hurdles against microorganisms by inhibiting their growth; these are essential control measures for particular foodborne pathogens. Different combinations of hurdles can be quantified and compared to each other in terms of their inhibitory effect ("iso-hurdle"). This paper presents a methodology for establishing microbial iso-hurdle rules in three steps: developing a predictive model based on existing but disparate datasets; building an experimental design focused on the iso-hurdles using the model output; and validating the model and the iso-hurdle rules with new data. The methodology is illustrated with Listeria monocytogenes. Existing data from industry, a public database and the literature were collected and analysed, after which a total of 650 growth rates were retained. A gamma-type model was developed for the factors temperature, pH, a(w), acetic, lactic and sorbic acids. Three iso-hurdle rules were assessed (40 logcount curves generated): salt replacement by addition of organic acids; sorbic acid replacement by addition of acetic and lactic acid; and sorbic acid replacement by addition of lactic/acetic acid and salt. For the three rules, the growth rates were equivalent in the whole experimental domain (γ from 0.1 to 0.5). The lag times were also equivalent in the case of mild inhibitory conditions (γ ≥ 0.2), while they were longer in the presence of salt than acids under stress conditions (γ < 0.2). This methodology allows an assessment of the equivalence of inhibitory effects without intensive data generation; it could be applied to develop milder formulations which guarantee microbial safety and stability. |
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Authors:
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Laure Pujol; Denis Kan-King-Yu; Yvan Le Marc; Moira D Johnston; Florence Rama-Heuzard; Sandrine Guillou; Peter McClure; Jeanne-Marie Membré |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-9 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: - ISSN: 1098-5336 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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INRA, UMR1014 Secalim, Nantes, F-44307, France. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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