| Establishing chitosan coated PLGA nanosphere platform loaded with wide variety of nucleic acid by complexation with cationic compound for gene delivery. | |
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MedLine Citation:
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PMID: 18178349 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this paper was to establish the surface modified poly(d,l-lactide-co-glycolide) (PLGA) nanosphere platform with chitosan (CS) for gene delivery by using the emulsion solvent diffusion (ESD) method. The advantages of this method are a simple process under mild conditions without sonication. This method requires essentially dissolving both polymer and drug in the organic solvent. Therefore a hydrophilic drug such as nucleic acid is hardly applied to the ESD method. Nucleic acid can easily form an ion-complex with cationic compound, which can be dissolved in the organic solvent. Thereafter, nucleic acid solubility for organic solution can increase by complexation with cationic compound. We used DOTAP as a cationic compound to increase the loading efficiency of nucleic acid. By coating the PLGA nanospheres with CS, the loading efficiency of nucleic acid in the modified nanospheres increased significantly. The release profile of nucleic acid from PLGA nanospheres exhibited sustained release after initial burst. The PLGA nanospheres coated with chitosan reduced the initial burst of nucleic acid release and prolonged the drugs releasing at later stage. Chitosan coated PLGA nanosphere platform was established to encapsulate satisfactorily wide variety of nucleic acid for an acceptable gene delivery system. |
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Authors:
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Kohei Tahara; Takeshi Sakai; Hiromitsu Yamamoto; Hirofumi Takeuchi; Yoshiaki Kawashima |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-11-09 |
Journal Detail:
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Title: International journal of pharmaceutics Volume: 354 ISSN: 0378-5173 ISO Abbreviation: Int J Pharm Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-03-24 Completed Date: 2008-07-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7804127 Medline TA: Int J Pharm Country: Netherlands |
Other Details:
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Languages: eng Pagination: 210-6 Citation Subset: IM |
Affiliation:
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Laboratory of Pharmaceutical Engineering, School of Pharmacy, Aichi Gakuin University, 1-100, Kusumoto, Chikusa, Nagoya, Aichi 464-8650, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chitosan
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chemistry DNA / chemistry* Delayed-Action Preparations Diffusion Emulsions / chemistry Fatty Acids, Monounsaturated / chemistry Gene Transfer Techniques* Lactic Acid / chemistry Nanospheres Oligonucleotides, Antisense / chemistry* Plasmids Polyglycolic Acid / chemistry Quaternary Ammonium Compounds / chemistry RNA, Small Interfering / chemistry* Solubility Solvents / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Delayed-Action Preparations; 0/Emulsions; 0/Fatty Acids, Monounsaturated; 0/Oligonucleotides, Antisense; 0/Quaternary Ammonium Compounds; 0/RNA, Small Interfering; 0/Solvents; 0/polylactic acid-polyglycolic acid copolymer; 113669-21-9/1,2-dioleoyloxy-3-(trimethylammonium)propane; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid; 9007-49-2/DNA; 9012-76-4/Chitosan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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