Document Detail


Establishing chitosan coated PLGA nanosphere platform loaded with wide variety of nucleic acid by complexation with cationic compound for gene delivery.
MedLine Citation:
PMID:  18178349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this paper was to establish the surface modified poly(d,l-lactide-co-glycolide) (PLGA) nanosphere platform with chitosan (CS) for gene delivery by using the emulsion solvent diffusion (ESD) method. The advantages of this method are a simple process under mild conditions without sonication. This method requires essentially dissolving both polymer and drug in the organic solvent. Therefore a hydrophilic drug such as nucleic acid is hardly applied to the ESD method. Nucleic acid can easily form an ion-complex with cationic compound, which can be dissolved in the organic solvent. Thereafter, nucleic acid solubility for organic solution can increase by complexation with cationic compound. We used DOTAP as a cationic compound to increase the loading efficiency of nucleic acid. By coating the PLGA nanospheres with CS, the loading efficiency of nucleic acid in the modified nanospheres increased significantly. The release profile of nucleic acid from PLGA nanospheres exhibited sustained release after initial burst. The PLGA nanospheres coated with chitosan reduced the initial burst of nucleic acid release and prolonged the drugs releasing at later stage. Chitosan coated PLGA nanosphere platform was established to encapsulate satisfactorily wide variety of nucleic acid for an acceptable gene delivery system.
Authors:
Kohei Tahara; Takeshi Sakai; Hiromitsu Yamamoto; Hirofumi Takeuchi; Yoshiaki Kawashima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-09
Journal Detail:
Title:  International journal of pharmaceutics     Volume:  354     ISSN:  0378-5173     ISO Abbreviation:  Int J Pharm     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-24     Completed Date:  2008-07-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7804127     Medline TA:  Int J Pharm     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  210-6     Citation Subset:  IM    
Affiliation:
Laboratory of Pharmaceutical Engineering, School of Pharmacy, Aichi Gakuin University, 1-100, Kusumoto, Chikusa, Nagoya, Aichi 464-8650, Japan.
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MeSH Terms
Descriptor/Qualifier:
Chitosan / chemistry
DNA / chemistry*
Delayed-Action Preparations
Diffusion
Emulsions / chemistry
Fatty Acids, Monounsaturated / chemistry
Gene Transfer Techniques*
Lactic Acid / chemistry
Nanospheres
Oligonucleotides, Antisense / chemistry*
Plasmids
Polyglycolic Acid / chemistry
Quaternary Ammonium Compounds / chemistry
RNA, Small Interfering / chemistry*
Solubility
Solvents / chemistry
Chemical
Reg. No./Substance:
0/Delayed-Action Preparations; 0/Emulsions; 0/Fatty Acids, Monounsaturated; 0/Oligonucleotides, Antisense; 0/Quaternary Ammonium Compounds; 0/RNA, Small Interfering; 0/Solvents; 0/polylactic acid-polyglycolic acid copolymer; 113669-21-9/1,2-dioleoyloxy-3-(trimethylammonium)propane; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid; 9007-49-2/DNA; 9012-76-4/Chitosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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