Document Detail


Established and novel methods of interrogating two-dimensional cell migration.
MedLine Citation:
PMID:  23038152     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The regulation of cell motility is central to living systems. Consequently, cell migration assays are some of the most frequently used in vitro assays. This article provides a comprehensive, detailed review of in vitro cell migration assays both currently in use and possible with existing technology. Emphasis is given to two-dimensional migration assays using densely organized cells such as the scratch assay. Assays are compared and categorized in an outline format according to their primary biological readout and physical parameters. The individual benefits of the various methods and quantification strategies are also discussed. This review provides an in-depth, structured overview of in vitro cell migration assays as a means of enabling the reader to make informed decisions among the growing number of options available for their specific cell migration application.
Authors:
William J Ashby; Andries Zijlstra
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review    
Journal Detail:
Title:  Integrative biology : quantitative biosciences from nano to macro     Volume:  4     ISSN:  1757-9708     ISO Abbreviation:  Integr Biol (Camb)     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-23     Completed Date:  2013-04-05     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  101478378     Medline TA:  Integr Biol (Camb)     Country:  England    
Other Details:
Languages:  eng     Pagination:  1338-50     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biophysical Phenomena
Cell Count
Cell Movement / physiology*
Cytological Techniques / methods
Electric Impedance
Magnetics
Microfluidic Analytical Techniques
Models, Biological
Systems Biology
Grant Support
ID/Acronym/Agency:
CA040035/CA/NCI NIH HHS; CA098131/CA/NCI NIH HHS; CA143081/CA/NCI NIH HHS; R01 CA143081/CA/NCI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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