Document Detail

Established mouse liver cell lines as a model system for studying epithelio-mesenchymal interactions in morphogenesis.
MedLine Citation:
PMID:  2386886     Owner:  NLM     Status:  MEDLINE    
Four cell lines were established from both fetal and adult livers of mice (ddY strain) by colony isolation methods, and these were characterized by morphological, immunological and biochemical examination. An epithelial cell line from a fetus (eE1-10) was identified to be derived from the biliary tract epithelium. Another epithelial cell line from an adult (aE3) reconstructed bile canaliculi-like structures in aggregates and secreted several serum proteins, indicating that it was derived from the hepatic parenchyma. The other two lines (eF1 and aF1) are fibroblastic in morphology. These were derived from fetal and adult livers, respectively. Both eE1-10 and the fetal fibroblastic cell line (eF1) are hypotetraploid, whereas both aE3 and the adult fibroblastic cell line (aF1) are hypodiploid. All four cell lines showed both contact inhibition and anchorage dependency of growth. Luminal structures with basal laminar, similar to bile ducts, were reconstituted from eE1-10 cells when co-aggregated with aF1 fibroblastic cells. Luminal structures were also formed from eE1-10 cells embedded in collagen gel. These, however, had no basal lamina and there was less development of microvilli than in aggregates mixed with fibroblastic cells. These results indicate that the fibroblastic cells afford favourable conditions for maintenance of the elaborated luminal structure of epithelial cells through production of the basal lamina.
Y Kadoya
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell differentiation and development : the official journal of the International Society of Developmental Biologists     Volume:  30     ISSN:  0922-3371     ISO Abbreviation:  Cell Differ. Dev.     Publication Date:  1990 May 
Date Detail:
Created Date:  1990-09-24     Completed Date:  1990-09-24     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8811335     Medline TA:  Cell Differ Dev     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  117-28     Citation Subset:  IM    
Institute of Molecular Biology, Faculty of Science, Nagoya University, Japan.
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MeSH Terms
Blood Proteins / metabolism
Cell Division
Cell Line
Epithelial Cells*
Epithelium / metabolism,  ultrastructure
Fibroblasts / cytology*,  metabolism,  ultrastructure
Liver / cytology*
Microscopy, Electron
Reg. No./Substance:
0/Blood Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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