Document Detail

Essential roles of Snf21, a Swi2/Snf2 family chromatin remodeler, in fission yeast mitosis.
MedLine Citation:
PMID:  19168987     Owner:  NLM     Status:  MEDLINE    
ATP-dependent chromatin remodelers (ADCRs) convert local chromatin structure into both transcriptional active and repressive state. Recent studies have revealed that ADCRs play diverse regulatory roles in chromosomal events such as DNA repair and recombination. Here we have newly identified a fission yeast gene encoding a Swi2/Snf2 family ADCR. The amino acid sequence of this gene, snf21(+), implies that Snf21 is a fission yeast orthologue of the budding yeast Sth1, the catalytic core of the RSC chromatin remodeling complex. The snf21(+) gene product is a nuclear protein essential to cell viability: the null mutant cells stop growing after several rounds of cell divisions. A temperature sensitive allele of snf21(+), snf21-36 exhibits at non-permissive temperature (34 degrees C) a cell cycle arrest at G2-M phase and defects in chromosome segregation, thereby causing cell elongation, lack of cell growth, and death of some cell population. snf21-36 shows thiabendazole (TBZ) sensitivity even at permissive temperature (25 degrees C). The TBZ sensitivity becomes severer as snf21-36 is combined with the deletion of a centromere-localized Mad2 spindle checkpoint protein. The cell cycle arrest phenotype at 34 degrees C cannot be rescued by the mad2(+) deletion, although it is substantially alleviated at 30 degrees C in mad2Delta. These data suggest that Snf21 plays an essential role in mitosis possibly functioning in centromeric chromatin.
Kentaro Yamada; Kouji Hirota; Ken-Ichi Mizuno; Takehiko Shibata; Kunihiro Ohta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genes & genetic systems     Volume:  83     ISSN:  1341-7568     ISO Abbreviation:  Genes Genet. Syst.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2009-01-26     Completed Date:  2009-03-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9607822     Medline TA:  Genes Genet Syst     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  361-72     Citation Subset:  IM    
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan.
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MeSH Terms
Cell Cycle Proteins / analysis,  genetics,  metabolism*
Cell Survival
Chromatin / metabolism
Chromatin Assembly and Disassembly / physiology*
Chromosome Segregation
DNA Helicases / genetics,  metabolism
Schizosaccharomyces / genetics*,  metabolism
Schizosaccharomyces pombe Proteins / analysis,  genetics,  metabolism*
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Chromatin; 0/Schizosaccharomyces pombe Proteins; EC 3.6.1.-/DNA Helicases; EC 3.6.1.-/Snf21 protein, S pombe

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