Document Detail


Essential and opposing roles of zebrafish beta-catenins in the formation of dorsal axial structures and neurectoderm.
MedLine Citation:
PMID:  16510506     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In Xenopus, Wnt signals and their transcriptional effector beta-catenin are required for the development of dorsal axial structures. In zebrafish, previous loss-of-function studies have not identified an essential role for beta-catenin in dorsal axis formation, but the maternal-effect mutation ichabod disrupts beta-catenin accumulation in dorsal nuclei and leads to a reduction of dorsoanterior derivatives. We have identified and characterized a second zebrafish beta-catenin gene, beta-catenin-2, located on a different linkage group from the previously studied beta-catenin-1, but situated close to the ichabod mutation on LG19. Although the ichabod mutation does not functionally alter the beta-catenin-2 reading frame, the level of maternal beta-catenin-2, but not beta-catenin-1, transcript is substantially lower in ichabod, compared with wild-type, embryos. Reduction of beta-catenin-2 function in wild-type embryos by injection of morpholino antisense oligonucleotides (MOs) specific for this gene (MO2) results in the same ventralized phenotypes as seen in ichabod embryos, and administration of MO2 to ichabod embryos increases the extent of ventralization. MOs directed against beta-catenin-1 (MO1), by contrast, had no ventralizing effect on wild-type embryos. beta-catenin-2 is thus specifically required for organizer formation and this function is apparently required maternally, because the ichabod mutation causes a reduction in maternal transcription of the gene and a reduced level of beta-catenin-2 protein in the early embryo. A redundant role of beta-catenins in suppressing formation of neurectoderm is revealed when both beta-catenin genes are inhibited. Using a combination of MO1 and MO2 in wild-type embryos, or by injecting solely MO1 in ichabod embryos, we obtain expression of a wide spectrum of neural markers in apparently appropriate anteroposterior pattern. We propose that the early, dorsal-promoting function of beta-catenin-2 is essential to counteract a later, dorsal- and neurectoderm-repressing function that is shared by both beta-catenin genes.
Authors:
Gianfranco Bellipanni; Máté Varga; Shingo Maegawa; Yoshiyuki Imai; Christina Kelly; Andrea Pomrehn Myers; Felicia Chu; William S Talbot; Eric S Weinberg
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2006-03-01
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  133     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-10     Completed Date:  2006-05-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  1299-309     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Pennsylvania, Philadelphia, 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Body Patterning / genetics*
Chromosome Mapping
Chromosomes
Computer Simulation
Embryo, Nonmammalian
Gene Expression Regulation, Developmental
Genetic Markers
Genome
Microinjections
Molecular Sequence Data
Nervous System / embryology*
Oligonucleotides, Antisense / pharmacology
Organizers, Embryonic / metabolism
Phenotype
Phylogeny
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Zebrafish / embryology*,  genetics
Zebrafish Proteins*
beta Catenin / chemistry,  genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
HD39272/HD/NICHD NIH HHS; RR12349/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Genetic Markers; 0/Oligonucleotides, Antisense; 0/RNA, Messenger; 0/Zebrafish Proteins; 0/beta Catenin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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