| Essential oil of Curcuma wenyujin induces apoptosis in human hepatoma cells. | |
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MedLine Citation:
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PMID: 18666318 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To investigate the effects of the essential oil of Curcuma wenyujin (CWO) on growth inhibition and on the induction of apoptosis in human HepG2 cancer cells. METHODS: The cytotoxic effect of drugs on HepG2 cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zolium bromide (MTT) assay. DNA fragmentation was visualized by agarose gel electrophoresis. Cell cycle and mitochondrial transmembrane potential (Delta psi m) were determined by flow cytometry (FCM). Cytochrome C immunostaining was evaluated by fluorescence microscopy. Caspase-3 enzymatic activity was assayed by the cleavage of Ac-DEVD-R110. Cleaved PARP and active caspase-3 protein levels were measured by FCM using BD(TM) CBA Human Apoptosis Kit. RESULTS: Treatment with CWO inhibited the growth of HepG2 cells in a dose-dependent manner, and the IC50 of CWO was approximately 70 mug/mL. CWO was found to inhibit the growth of HepG2 cells by inducing a cell cycle arrest at S/G(2). DNA fragmentation was evidently observed at 70 mug/mL after 72 h of treatment. During the process, cytosolic HepG2 cytochrome C staining showed a markedly stronger green fluorescence than in control cells in a dose-dependent fashion, and CWO also caused mitochondrial transmembrane depolarization. Furthermore, the results clearly demonstrated that both, activity of caspase-3 enzyme and protein levels of cleaved PARP, significantly increased in a dose-dependent manner after treatment with CWO. CONCLUSION: CWO exhibits an antiproliferative effect in HepG2 cells by inducing apoptosis. This growth inhibition is associated with cell cycle arrest, cytochrome C translocation, caspase 3 activation, Poly-ADP-ribose polymerase (PARP) degradation, and loss of mitochondrial membrane potential. This process involves a mitochondria-caspase dependent apoptosis pathway. As apoptosis is an important anti-cancer therapeutic target, these results suggest a potential of CWO as a chemotherapeutic agent. |
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Authors:
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Yu Xiao; Feng-Qing Yang; Shao-Ping Li; Guang Hu; Simon-Ming-Yuen Lee; Yi-Tao Wang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 14 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-07-30 Completed Date: 2008-11-13 Revised Date: 2010-09-22 |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 4309-18 Citation Subset: IM |
Affiliation:
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Institute of Chinese Medical Sciences, University of Macau, Av. Padre Tomas Pereira, SJ, Taipa, Macao, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Carcinoma, Hepatocellular / embryology* Caspase 3 / metabolism Cell Cycle Cell Line Cell Line, Tumor Curcuma / metabolism* Cytochromes c / chemistry Humans Liver Neoplasms / metabolism* Membrane Potentials Microscopy, Fluorescence Mitochondrial Membranes / metabolism Oils, Volatile / metabolism* Plant Extracts / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Oils, Volatile; 0/Plant Extracts; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspase 3 |
| Comments/Corrections | |
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