| Essential fatty acid uptake and esterification in primary culture of rat hepatocytes. | |
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MedLine Citation:
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PMID: 3092869 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Primary cultures of adult rat hepatocytes were used to compare the uptake and esterification of essential polyunsaturated fatty acids (18:2, 20:3 and 20:4 of the n-6 series) with those of palmitic and oleic acids. The uptake of unesterified fatty acids was linearly related to the free fatty acid/albumin molar ratio for 14 h and did not depend on the unbound free fatty acid level. Whatever the initial free fatty acid/albumin molar ratio, it dropped to 0.5 +/- 0.1 mM after 14 h, thus showing that hepatocytes have a high capacity for clearing free fatty acids from the medium at high free fatty acid/albumin molar ratios. The free fatty acid uptake become saturable when the free fatty acid and albumin concentrations were raised and the free fatty acid/albumin ratio remained constant. This strongly suggests that albumin-hepatocyte interaction mediates free fatty acid uptake. This uptake was identical whatever the fatty acid tested and did not depend on the relative amounts of fatty acids when they were added simultaneously. Triacylglycerol accumulation and synthesis, monitored by labelled fatty acids, were related to the free fatty acid/albumin molar ratio and exhibited no specificity for the series of fatty acids tested. Triacylglycerols were enriched in all the fatty acids tested by up to 60%, and fatty acid incorporation into diacylglycerols and triacylglycerols reflected the free fatty acid composition of the medium. By contrast, neither the level nor the synthesis of phospholipids varied with free fatty acid/albumin, but the rate of phospholipid turnover depended on the fatty acids tested. Accumulation of these acids was smaller in phospholipids than in triacylglycerols. When linoleic and arachidonic acids were added together, phospholipids (especially phosphatidylethanolamine and phosphatidylinositol) were more enriched in arachidonic acid than triacylglycerols. This might be due to the specificity for fatty acid of the enzymes involved in phospholipid metabolism. |
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Authors:
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J Chambaz; A Guillouzo; P Cardot; D Pepin; G Bereziat |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 878 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1986 Oct |
Date Detail:
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Created Date: 1986-11-12 Completed Date: 1986-11-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 310-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid Arachidonic Acids / metabolism Cells, Cultured Esterification Fatty Acids, Essential / metabolism* Fatty Acids, Nonesterified / metabolism Linoleic Acid Linoleic Acids / metabolism Liver / metabolism* Male Oleic Acid Oleic Acids / metabolism Palmitic Acid Palmitic Acids / metabolism Phospholipids / metabolism Rats Rats, Inbred Strains Serum Albumin / metabolism Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Fatty Acids, Essential; 0/Fatty Acids, Nonesterified; 0/Linoleic Acids; 0/Oleic Acids; 0/Palmitic Acids; 0/Phospholipids; 0/Serum Albumin; 0/Triglycerides; 112-80-1/Oleic Acid; 2197-37-7/Linoleic Acid; 506-32-1/Arachidonic Acid; 57-10-3/Palmitic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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