| Essential fatty acid deficiency in mice impairs lactose digestion. | |
| | |
MedLine Citation:
|
PMID: 18653724 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Essential fatty acid (EFA) deficiency in mice induces fat malabsorption. We previously reported indications that the underlying mechanism is located at the level of the intestinal mucosa. We have investigated the effects of EFA deficiency on small intestinal morphology and function. Mice were fed an EFA-deficient or control diet for 8 wk. A 72-h fat balance, the EFA status, and small intestinal histology were determined. Carbohydrate absorptive and digestive capacities were assessed by stable isotope methodology after administration of [U-(13)C]glucose and [1-(13)C]lactose. The mRNA expression and enzyme activity of lactase, and concentrations of the EFA linoleic acid (LA) were measured in small intestinal mucosa. Mice fed the EFA-deficient diet were markedly EFA-deficient with a profound fat malabsorption. EFA deficiency did not affect the histology or proliferative capacity of the small intestine. Blood [13C6]glucose appearance and disappearance were similar in both groups, indicating unaffected monosaccharide absorption. In contrast, blood appearance of [13C]glucose, originating from [1-(13)C]lactose, was delayed in EFA-deficient mice. EFA deficiency profoundly reduced lactase activity (-58%, P<0.01) and mRNA expression (-55%, P<0.01) in mid-small intestine. Both lactase activity and its mRNA expression strongly correlated with mucosal LA concentrations (r=0.77 and 0.79, respectively, P<0.01). EFA deficiency in mice inhibits the capacity to digest lactose but does not affect small intestinal histology. These data underscore the observation that EFA deficiency functionally impairs the small intestine, which in part may be mediated by low LA levels in the enterocytes. |
| | |
Authors:
|
S Lukovac; E L Los; F Stellaard; E H H M Rings; H J Verkade |
Related Documents
:
|
7883744 - Changes in ornithine metabolic enzymes induced by dietary protein in small intestine an... 19556794 - Analysis of fat digestive and absorptive function after subtotal gastrectomy by a 13c-l... 1649804 - Influence of intestinal resection and type of diet on digestive utilization and metabol... 17638014 - Morphological changes of the rat intestinal lining in relation to body stores depletion... 17526664 - Influence of surfactant supplementation and maceration on the feeding value of rice str... 21865564 - Longitudinal change in diet quality in australian adults varies by demographic, socio-e... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-24 |
Journal Detail:
|
Title: American journal of physiology. Gastrointestinal and liver physiology Volume: 295 ISSN: 0193-1857 ISO Abbreviation: Am. J. Physiol. Gastrointest. Liver Physiol. Publication Date: 2008 Sep |
Date Detail:
|
Created Date: 2008-09-10 Completed Date: 2008-10-09 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100901227 Medline TA: Am J Physiol Gastrointest Liver Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: G605-13 Citation Subset: IM |
Affiliation:
|
Pediatric Gastroenterology, Department of Pediatrics, Beatrix Children's Hospital, Groningen University Institute for Drug Exploration, Center for Liver, Digestive and Metabolic Diseases, Groningen, The Netherlands. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood Glucose / metabolism Carbon Isotopes Dietary Fats / metabolism* Digestion* Down-Regulation Fatty Acids, Essential / deficiency* Glucose / administration & dosage, metabolism* Intestinal Absorption* Intestine, Small / enzymology, metabolism* Kinetics Lactase / genetics, metabolism Lactose / administration & dosage, blood, metabolism* Lactose Intolerance / etiology, metabolism*, physiopathology Linoleic Acid / metabolism Male Mice RNA, Messenger / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Blood Glucose; 0/Carbon Isotopes; 0/Dietary Fats; 0/Fatty Acids, Essential; 0/RNA, Messenger; 2197-37-7/Linoleic Acid; 50-99-7/Glucose; 63-42-3/Lactose; EC 3.2.1.108/Lactase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The role of luminal factors in the recovery of gastric function and behavioral changes after chronic...
Next Document: Phenylalanine and tyrosine kinetics in compensated liver cirrhosis: effects of meal ingestion.