| Essential factors in the kinetic analysis of RNA synthesis in HeLa cells. | |
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MedLine Citation:
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PMID: 853064 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Further evidence of mRNA in HeLa cells with a half-life two hours or less is given. A kinetic model of RNA synthesis in HeLa cells is described in which equilibration of label occurs first into the acid soluble pool (evidence is given that this pool feeds RNA synthesis) and thence in nuclear and cytoplasmic molecules. The measured accumulation of label in nuclear and cytoplasmic poly (A) is examined with the model and parameters were found which are consistent with the quantitative transfer of nuclear poly(A) to the cytoplasm. The strengths and limitations of the model are discussed. |
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Authors:
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L Puckett; J E Darnell |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular physiology Volume: 90 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1977 Mar |
Date Detail:
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Created Date: 1977-06-11 Completed Date: 1977-06-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 521-34 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Nucleus
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metabolism Cytoplasm / metabolism Half-Life Hela Cells / metabolism* Humans Models, Biological Poly A / biosynthesis, metabolism RNA, Messenger / biosynthesis RNA, Neoplasm / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/RNA, Neoplasm; 24937-83-5/Poly A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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