| Essential roles of androgen signaling in Wolffian duct stabilization and epididymal cell differentiation. | |
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MedLine Citation:
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PMID: 21303954 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The epididymis is a male accessory organ and functions for sperm maturation and storage under the control of androgen. The development of the epididymis is also androgen dependent. The Wolffian duct (WD), anlagen of the epididymis, is formed in both male and female embryos; however, it is stabilized only in male embryos by testicular androgen. Androgen drives subsequent differentiation of the WD into the epididymis. Although the essential roles of androgen in WD masculinization and epididymal function have been established, little is known about cellular events regulated precisely by androgen signaling during these processes. It is also unclear whether androgen signaling, especially in the epithelia, has further function for epididymal epithelial cell differentiation. In this study we examined the cellular death and proliferation controlled by androgen signaling via the androgen receptor (AR) in WD stabilization. Analyses using AR knockout mice revealed that androgen signaling inhibits epithelial cell death in this process. Analysis of AP2α-Cre;AR(flox/Y) mice, in which AR function is deleted in the WD epithelium, revealed that epithelial AR is not required for the WD stabilization but is required for epithelial cell differentiation in the epididymis. Specifically, loss of epithelial AR significantly reduced expression of p63 that is essential for differentiation of basal cells in the epididymal epithelium. We also interrogated the possibility of regulation of the p63 gene (Trp63) by AR in vitro and found that p63 is a likely direct target of AR regulation. |
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Authors:
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Aki Murashima; Shinichi Miyagawa; Yukiko Ogino; Hisayo Nishida-Fukuda; Kimi Araki; Takahiro Matsumoto; Takehito Kaneko; Kazuya Yoshinaga; Ken-ichi Yamamura; Takeshi Kurita; Shigeaki Kato; Anne M Moon; Gen Yamada |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-02-08 |
Journal Detail:
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Title: Endocrinology Volume: 152 ISSN: 1945-7170 ISO Abbreviation: Endocrinology Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-24 Completed Date: 2011-05-23 Revised Date: 2012-10-30 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 1640-51 Citation Subset: AIM; IM |
Affiliation:
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Institute of Molecular Embryology and Genetics, Graduate School of Medical and Pharmaceutical Sciences, Global Center of Excellence Cell Fate Regulation Research and Education Unit, Kumamoto University, Kumamoto 860-0811, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / genetics, physiology Cell Differentiation / genetics, physiology Cell Proliferation Epididymis / cytology*, embryology, transplantation Female Immunohistochemistry Male Mice Mice, Knockout Mice, Nude Phosphoproteins / genetics, metabolism* Pregnancy Receptors, Androgen / genetics, metabolism* Reverse Transcriptase Polymerase Chain Reaction Trans-Activators / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA154358/CA/NCI NIH HHS; R01 HD046767-02/HD/NICHD NIH HHS; R01 HD046767-03/HD/NICHD NIH HHS; R01 HD046767-04/HD/NICHD NIH HHS; R01CA154358-01/CA/NCI NIH HHS; R01ES016597-01A1/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phosphoproteins; 0/Receptors, Androgen; 0/Trans-Activators; 0/Trp63 protein, mouse |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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