| Escherichia coli inactivation mechanism by pressurized CO2. | |
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MedLine Citation:
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PMID: 17473890 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of pressurized CO2 on the survival of Escherichia coli and the mechanism of cell inactivation were studied. Bacterial cultures were inoculated in nutrient broth and incubated at 30 degrees C for 18 h. Exposure of the cells to CO2 under pressures ranging from 2.5 to 25 MPa and at temperatures between 8 and 40 degrees C was performed in a double-walled reactor with a 1 L capacity. The effect of the treatment on the cells was evaluated by plating and by transmission and scanning electron microscopy observation. Vapour CO2 generated a bacteriostatic effect. In liquid or supercritical state, CO2 provided a bactericidal effect. The bactericidal effect increased with pressure and temperature. The mechanism of cell inactivation by liquid CO2 involved two stages. First, cell stress caused by the CO2 penetration provoked cell wall collapse and cellular content precipitation. Second, the cell death caused by supercritical extraction of intracellular substances and cell envelope perforation resulted in leaking of intracellular constituents. In supercritical conditions, the cell inactivation process had one single phase: cellular death. |
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Authors:
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Mathias K Oulé; Kablan Tano; Anne-Marie Bernier; Joseph Arul |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Canadian journal of microbiology Volume: 52 ISSN: 0008-4166 ISO Abbreviation: Can. J. Microbiol. Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2007-05-02 Completed Date: 2007-08-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372707 Medline TA: Can J Microbiol Country: Canada |
Other Details:
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Languages: eng Pagination: 1208-17 Citation Subset: IM |
Affiliation:
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Faculty of Science, Collège universitaire de Saint-Boniface, Winnipeg, Canada. moule@ustboniface.mb.ca |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbon Dioxide
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pharmacology* Culture Media / pharmacology Escherichia coli / drug effects*, growth & development, ultrastructure Microscopy, Electron, Scanning Microscopy, Electron, Transmission Pressure Temperature |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 124-38-9/Carbon Dioxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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