Document Detail


Escherichia coli inactivation mechanism by pressurized CO2.
MedLine Citation:
PMID:  17473890     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of pressurized CO2 on the survival of Escherichia coli and the mechanism of cell inactivation were studied. Bacterial cultures were inoculated in nutrient broth and incubated at 30 degrees C for 18 h. Exposure of the cells to CO2 under pressures ranging from 2.5 to 25 MPa and at temperatures between 8 and 40 degrees C was performed in a double-walled reactor with a 1 L capacity. The effect of the treatment on the cells was evaluated by plating and by transmission and scanning electron microscopy observation. Vapour CO2 generated a bacteriostatic effect. In liquid or supercritical state, CO2 provided a bactericidal effect. The bactericidal effect increased with pressure and temperature. The mechanism of cell inactivation by liquid CO2 involved two stages. First, cell stress caused by the CO2 penetration provoked cell wall collapse and cellular content precipitation. Second, the cell death caused by supercritical extraction of intracellular substances and cell envelope perforation resulted in leaking of intracellular constituents. In supercritical conditions, the cell inactivation process had one single phase: cellular death.
Authors:
Mathias K Oulé; Kablan Tano; Anne-Marie Bernier; Joseph Arul
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  52     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2007-05-02     Completed Date:  2007-08-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  1208-17     Citation Subset:  IM    
Affiliation:
Faculty of Science, Collège universitaire de Saint-Boniface, Winnipeg, Canada. moule@ustboniface.mb.ca
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MeSH Terms
Descriptor/Qualifier:
Carbon Dioxide / pharmacology*
Culture Media / pharmacology
Escherichia coli / drug effects*,  growth & development,  ultrastructure
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Pressure
Temperature
Chemical
Reg. No./Substance:
0/Culture Media; 124-38-9/Carbon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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