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Escherichia coli O157:H7 Populations in Ruminants Can Be Reduced by Orange Peel Product Feeding.
MedLine Citation:
PMID:  22054194     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Foodborne pathogenic bacteria such as Escherichia coli O157:H7 are threats to the safety of beef. Citrus peel and dried orange pulp are by-products from citrus juice production that have natural antimicrobial effects and are often incorporated into least-cost ration formulations for beef and dairy cattle. This study was designed to determine if orange peel and pulp affected E. coli O157:H7 populations in vivo. Sheep (n = 24) were fed a cracked corn grain-based diet that was supplemented with a 50-50 mixture of dried orange pellet and fresh orange peel to achieve a final concentration (dry matter basis, wt/wt) of 0, 5, or 10% pelleted orange peel (OP) for 10 days. Sheep were artificially inoculated with 10(10) CFU of E. coli O157:H7 by oral dosing. Fecal shedding of E. coli O157:H7 was measured daily for 5 days after inoculation, after which all animals were humanely euthanized. At 96 h postinoculation, E. coli O157:H7 shedding was reduced (P < 0.05) in sheep fed 10% OP. Populations of inoculated E. coli O157:H7 were reduced by OP treatment throughout the gastrointestinal tract; however, this reduction reached significant levels in the rumen (P < 0.05) of sheep fed 10% OP diets. Cecal and rectal populations of E. coli O157:H7 were reduced (P < 0.05) by inclusion of both 5 and 10% OP diets. Our results demonstrate that orange peel products can be used as a preharvest intervention strategy as part of an integrated pathogen reduction scheme.
Authors:
Todd R Callaway; Jeffery A Carroll; John D Arthington; Tom S Edrington; Michelle L Rossman; Mandy A Carr; Nathan A Krueger; Steven C Ricke; Phil Crandall; David J Nisbet
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  74     ISSN:  1944-9097     ISO Abbreviation:  J. Food Prot.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1917-21     Citation Subset:  IM    
Affiliation:
Food and Feed Safety Research Unit, U.S. Department of Agriculture, Agricultural Research Service, College Station, Texas 77845, USA. todd.callaway@ars.usda.gov.
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