| An Escherichia coli K-12 tktA tktB mutant deficient in transketolase activity requires pyridoxine (vitamin B6) as well as the aromatic amino acids and vitamins for growth. | |
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MedLine Citation:
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PMID: 7928977 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We show that a tktA tktB double mutant, which is devoid of the two known transketolase isoenzymes of Escherichia coli K-12, requires pyridoxine (vitamin B6) as well as the aromatic amino acids and vitamins for growth. This pyridoxine requirement can also be satisfied by 4-hydroxy-L-threonine or glycolaldehyde. These results provide direct evidence that D-erythrose-4-phosphate is a precursor of the pyridine ring of pyridoxine. In addition, they show that the two major E. coli transketolase isoenzymes are not required for the biosynthesis of D-1-deoxyxylulose, which is thought to be another precursor of pyridoxine. |
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Authors:
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G Zhao; M E Winkler |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 176 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1994 Oct |
Date Detail:
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Created Date: 1994-10-28 Completed Date: 1994-10-28 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 6134-8 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston 77030-1501. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism* Escherichia coli / enzymology, genetics, growth & development* Genes, Bacterial / genetics Isoenzymes / genetics, metabolism Mutation Pyridoxine / metabolism* Transketolase / genetics, metabolism* Vitamins / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01-GM37561/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Isoenzymes; 0/Vitamins; 65-23-6/Pyridoxine; EC 2.2.1.1/Transketolase |
| Comments/Corrections | |
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