Document Detail


An Escherichia coli K-12 tktA tktB mutant deficient in transketolase activity requires pyridoxine (vitamin B6) as well as the aromatic amino acids and vitamins for growth.
MedLine Citation:
PMID:  7928977     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We show that a tktA tktB double mutant, which is devoid of the two known transketolase isoenzymes of Escherichia coli K-12, requires pyridoxine (vitamin B6) as well as the aromatic amino acids and vitamins for growth. This pyridoxine requirement can also be satisfied by 4-hydroxy-L-threonine or glycolaldehyde. These results provide direct evidence that D-erythrose-4-phosphate is a precursor of the pyridine ring of pyridoxine. In addition, they show that the two major E. coli transketolase isoenzymes are not required for the biosynthesis of D-1-deoxyxylulose, which is thought to be another precursor of pyridoxine.
Authors:
G Zhao; M E Winkler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  176     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1994-10-28     Completed Date:  1994-10-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6134-8     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston 77030-1501.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Escherichia coli / enzymology,  genetics,  growth & development*
Genes, Bacterial / genetics
Isoenzymes / genetics,  metabolism
Mutation
Pyridoxine / metabolism*
Transketolase / genetics,  metabolism*
Vitamins / metabolism
Grant Support
ID/Acronym/Agency:
R01-GM37561/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Isoenzymes; 0/Vitamins; 65-23-6/Pyridoxine; EC 2.2.1.1/Transketolase
Comments/Corrections

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