Document Detail

Escherichia coli Inactivation by UVC-Irradiated C60: Kinetics and Mechanisms.
MedLine Citation:
PMID:  21999435     Owner:  NLM     Status:  Publisher    
Motivated by recent studies that documented changes in fullerene toxicity after chemical transformation, C60 aggregates (nC60) were subject to UVC irradiation at monochromatic 254 nm and subsequently evaluated for antibacterial and bactericidal properties against Escherichia coli. The nC60 treated with UVC irradiation, referred to herein as UVC-irradiated C60, did not directly inhibit bacterial growth at concentrations up to 20 mg/L. In the presence of UVA and visible light, however, UVC-irradiated C60 rapidly inactivated E. coli, suggesting that photochemical production of reactive oxygen species (ROS) was involved. The use of ROS scavengers and probes determined that hydroxyl radicals were the primary ROS responsible for the E. coli inactivation. Results from protein release, lipid peroxidation, cell permeability and intracellular enzyme assays suggest that the inactivation mechanism involves UVC-irradiated C60 diffusing through E. coli cell membrane and producing hydroxyl radicals within the cell. Further study on water soluble C60 derivatives and possible transformative processes is, therefore, recommended based on the environmental implications of results presented herein that nC60 exposed to UVC irradiation is more toxic than parent nC60.
Min Cho; Samuel D Snow; Joseph B Hughes; Jaehong Kim
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-15
Journal Detail:
Title:  Environmental science & technology     Volume:  -     ISSN:  1520-5851     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0213155     Medline TA:  Environ Sci Technol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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