Document Detail


Erythropoietin promotes hippocampal neurogenesis in in vitro models of neonatal stroke.
MedLine Citation:
PMID:  20117210     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The hippocampus is often injured in neonatal stroke. We have investigated the effect of erythropoietin (EPO) on oxygen-glucose deprived hippocampal slices and hypoxic progenitor cells. EPO improved survival of the organotypic hippocampal slices with significantly less cell death in the dentate gyrus and an increased number of proliferating cells 4-5 days after insult. Significantly fewer markers of neurogenesis were seen after the insult but when EPO was added to the culture medium, neurogenesis was sustained. When hippocampal progenitor cultures were stimulated into differentiation, more cells chose a neuronal cell fate when treated with EPO. These findings support the hypothesis that EPO not only prevents ischemia induced cell death but promotes neuronal cell fate commitment in in vitro models of neonatal stroke.
Authors:
Damjan Osredkar; Jeffrey W Sall; Philip E Bickler; Donna M Ferriero
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-02-01
Journal Detail:
Title:  Neurobiology of disease     Volume:  38     ISSN:  1095-953X     ISO Abbreviation:  Neurobiol. Dis.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-12     Completed Date:  2010-07-01     Revised Date:  2014-09-09    
Medline Journal Info:
Nlm Unique ID:  9500169     Medline TA:  Neurobiol Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  259-65     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Blotting, Western
Cell Hypoxia / drug effects
Cell Proliferation / drug effects*
Cell Survival / drug effects*
Cells, Cultured
Erythropoietin / metabolism,  pharmacology*
Glucose / deficiency
Hippocampus / cytology,  drug effects*,  metabolism
Immunohistochemistry
Neurogenesis / drug effects*
Neurons / cytology,  drug effects,  metabolism
Organ Culture Techniques
Rats
Rats, Sprague-Dawley
Receptors, Erythropoietin / metabolism
Stem Cells
Grant Support
ID/Acronym/Agency:
K08 GM086511/GM/NIGMS NIH HHS; P50 NS035902/NS/NINDS NIH HHS; P50 NS035902-12/NS/NINDS NIH HHS; P50NS35902/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Erythropoietin; 11096-26-7/Erythropoietin; IY9XDZ35W2/Glucose
Comments/Corrections

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