Document Detail


Erythropoietin is equally effective as fresh-blood transfusion at reducing infarct size in anemic rats.
MedLine Citation:
PMID:  20693887     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: We recently demonstrated that transfusion of anemic animals up to 100 g/L hemoglobin with fresh blood protects the heart from ischemic injuries following myocardial infarction. Erythropoietin has cardioprotective effects independent of its erythropoietic activity. The objective of this study was to compare the cardioprotective effects of erythropoietin treatment to fresh-blood transfusion in anemic rats after acute myocardial infarction.
DESIGN: Randomized animal study.
SETTING: University laboratory.
SUBJECTS: Male Sprague-Dawley rats weighing 200-300 g.
INTERVENTION: Myocardial infarction was induced by coronary artery ligation in 76 rats, 55 of which were anemic (80-90 g/L) and 21 of which had normal hemoglobin levels. Animals were randomized to erythropoietin (2000 units/kg), fresh-blood transfusion to 100 g/L hemoglobin, or saline-treatment groups immediately following myocardial infarction.
MEASUREMENTS AND MAIN RESULTS: At 24 hrs after myocardial infarction, cardiac function and infarct size were determined. Myocardial apoptosis was determined by caspase-3 activity and terminal deoxynucleotidyl transferase d-UTP nick end labeling (TUNEL) assay. Infarct size was significantly decreased in anemic rats treated with erythropoietin or blood transfusion compared to those in the saline-treatment group. Cardiac function, as measured by maximal positive and minimal negative first derivatives of left ventricular pressure, was better preserved in the normal hemoglobin groups and the erythropoietin- or transfusion-treated anemic animals compared to saline-treated anemic animals. Myocardial caspase-3 activity and TUNEL-positive nuclei were significantly increased in anemic rats but were decreased by erythropoietin treatment or red blood cell transfusion.
CONCLUSIONS: Erythropoietin treatment is equally effective as fresh-blood transfusion in anemic rats after acute myocardial infarction at reducing infarct size, myocardial apoptosis, and improving cardiac function.
Authors:
Anargyros Xenocostas; Houxiang Hu; Nicolas Chin-Yee; Xiangru Lu; Ian Chin-Yee; Qingping Feng
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Critical care medicine     Volume:  38     ISSN:  1530-0293     ISO Abbreviation:  Crit. Care Med.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-20     Completed Date:  2010-11-02     Revised Date:  2011-01-07    
Medline Journal Info:
Nlm Unique ID:  0355501     Medline TA:  Crit Care Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2215-21     Citation Subset:  AIM; IM    
Affiliation:
Centre for Critical Illness, Research Lawson Health Research Institute, Canadian Blood Services, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Anemia / complications*,  drug therapy,  therapy
Animals
Apoptosis
Blood Pressure / physiology
Blood Transfusion*
Cardiotonic Agents / therapeutic use*
Caspase 3 / metabolism
Erythropoietin / therapeutic use*
Heart / physiopathology
Heart Rate / physiology
Hemoglobins / analysis
In Situ Nick-End Labeling
Male
Myocardial Infarction / complications,  drug therapy*,  physiopathology,  therapy
Myocardium / enzymology
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Cardiotonic Agents; 0/Hemoglobins; 11096-26-7/Erythropoietin; EC 3.4.22.-/Caspase 3
Comments/Corrections
Erratum In:
Crit Care Med. 2011 Jan;39(1):243

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