Document Detail


Erythropoietin effects on iron metabolism in rat bone marrow cells.
MedLine Citation:
PMID:  1125327     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper describes a study of the incorporation of 59-Fe from 59-Fe-labelled rat transferrin into rat bone marrow cells in culture. 59-Fe was found in both stroma and cytoplasm of marrow cells, and the cytoplasmic 59-Fe separated by polyacrylamide gel electrophoresis, into ferritin, haemoglobin and a low molecular weight fraction. The incorporation of 59-Fe into all three cytoplasmic fractions, but not into the stroma, increased progressively with time. Erythropoietin stimulated the increase of 59-Fe in ferritin within 1 h, the earliest time examined, and more than 3 h later in the stroma and haemoglobin. A proportion of the 59-Fe incorporated into the stroma and low molecular weight iron fractions during a 1 h incubation with 59-Fe-labelled transferrin was mobilised into ferritin and haemoglobin during a subsequent 4-h "cold-chase". Erythropoietin, when present during the "cold-chase", did not influence these 59-Fe fluxes. The erythropoietin stimulation of 59-Fe incorporation into ferritin, one of the earliest erythropoietin effects to be recorded, was therefore considered to be due to an increase of 59-Fe uptake by the hormone-responsive cells rather than a direct effect on ferritin synthesis. 20-h cultures containing erythropoietin when incubated with 59-Fe-labelled transferrin for 4 h, showed dose-related erythropoietin stimulation of 59-Fe incorporation into haemoglobin only. In the presence of 10 mM isonicotinic acid hydrazide, 59-Fe incorporation into haemoglobin was inhibited, as in reticulocytes (Ponka, P. and Neuwrit, J. (1969) Blood 33, 690-707), while that into the stroma, ferritin and low molecular weight iron fractions, was stimulated; there were no reproducible effects of erythropoietin.
Authors:
P L Storring; S Fatih
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  392     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1975 May 
Date Detail:
Created Date:  1975-08-04     Completed Date:  1975-08-04     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  26-38     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow / drug effects,  metabolism*
Bone Marrow Cells*
Cells, Cultured
Cytoplasm / metabolism
Electrophoresis, Polyacrylamide Gel
Erythropoietin / pharmacology*
Iron / metabolism*
Kinetics
Protein Binding
Rabbits / immunology
Rats
Time Factors
Transferrin / metabolism
Chemical
Reg. No./Substance:
11096-26-7/Erythropoietin; 11096-37-0/Transferrin; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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