Document Detail

Erythropoietin effects on iron metabolism in rat bone marrow cells.
MedLine Citation:
PMID:  1125327     Owner:  NLM     Status:  MEDLINE    
This paper describes a study of the incorporation of 59-Fe from 59-Fe-labelled rat transferrin into rat bone marrow cells in culture. 59-Fe was found in both stroma and cytoplasm of marrow cells, and the cytoplasmic 59-Fe separated by polyacrylamide gel electrophoresis, into ferritin, haemoglobin and a low molecular weight fraction. The incorporation of 59-Fe into all three cytoplasmic fractions, but not into the stroma, increased progressively with time. Erythropoietin stimulated the increase of 59-Fe in ferritin within 1 h, the earliest time examined, and more than 3 h later in the stroma and haemoglobin. A proportion of the 59-Fe incorporated into the stroma and low molecular weight iron fractions during a 1 h incubation with 59-Fe-labelled transferrin was mobilised into ferritin and haemoglobin during a subsequent 4-h "cold-chase". Erythropoietin, when present during the "cold-chase", did not influence these 59-Fe fluxes. The erythropoietin stimulation of 59-Fe incorporation into ferritin, one of the earliest erythropoietin effects to be recorded, was therefore considered to be due to an increase of 59-Fe uptake by the hormone-responsive cells rather than a direct effect on ferritin synthesis. 20-h cultures containing erythropoietin when incubated with 59-Fe-labelled transferrin for 4 h, showed dose-related erythropoietin stimulation of 59-Fe incorporation into haemoglobin only. In the presence of 10 mM isonicotinic acid hydrazide, 59-Fe incorporation into haemoglobin was inhibited, as in reticulocytes (Ponka, P. and Neuwrit, J. (1969) Blood 33, 690-707), while that into the stroma, ferritin and low molecular weight iron fractions, was stimulated; there were no reproducible effects of erythropoietin.
P L Storring; S Fatih
Related Documents :
7227667 - Influence of fucoidan on the intestinal absorption of iron, cobalt, manganese and zinc ...
16828857 - Acoustic startle response is disrupted in iron-deficient rats.
8294107 - Pathways of intracellular trafficking and release of ferritin by the liver in vivo: the...
18555617 - Psychological stress induces dysregulation of iron metabolism in rat brain.
6796767 - Possible explanation for hyperglycinaemia in propionic acidaemia and methylmalonic acid...
11780197 - Recording of spared motor evoked potentials and its augmentation by 4-aminopyridine in ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  392     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1975 May 
Date Detail:
Created Date:  1975-08-04     Completed Date:  1975-08-04     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  26-38     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bone Marrow / drug effects,  metabolism*
Bone Marrow Cells*
Cells, Cultured
Cytoplasm / metabolism
Electrophoresis, Polyacrylamide Gel
Erythropoietin / pharmacology*
Iron / metabolism*
Protein Binding
Rabbits / immunology
Time Factors
Transferrin / metabolism
Reg. No./Substance:
11096-26-7/Erythropoietin; 11096-37-0/Transferrin; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A novel method for the follow-up of shape changes in erythrocyte and other particles.
Next Document:  Rapid uptake by liver sinusoidal cells of serum albumin modified with retention of its compact confo...