| Erythropoietin can promote survival of cerebral cells by downregulating Bax gene after traumatic brain injury in rats. | |
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MedLine Citation:
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PMID: 20139499 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Traumatic brain injury (TBI) is an important cause of adult mortality and morbidity. Erythropoietin (Epo) has been shown to promote the viability of cerebral cells by upregulating Bcl-2 gene; however, Epo may exert its antiapoptotic effect via the differential regulation of the expression of genes involved in the apoptotic process. AIM: The present study examined the neuroprotective effect of Epo as a survival factor through the regulation of the Bax. MATERIALS AND METHODS: Wistar rats were randomly divided into three groups: Recombinant human EPO treated (rhEPO) TBI, vehicle-treated TBI, and sham-operated. Traumatic brain injury was induced by the Feeney free-falling model. Rats were killed 5, 12, 24, 72, 120, or 168 h after TBI. Regulation of Bcl-2 was detected by reverse transcription-polymerase chain reaction (RT-PCR), western blotting and immunofluorescence. RESULTS: Bax mRNA and protein levels were lower in the rhEPO)-treated rat brains than in the vehicle-treated rat brains. Induction of Bax expression peaked at 24 h and remained stable for 72-120 h in vehicle-treated rat brains, whereas induction of Bax expression was only slightly elevated in rhEPO-treated rat brains. The number of TdT-mediated dUTP Nick-End Labeling(TUNEL)-positive cells in the rhEPO-treated rat brains was far fewer than in the vehicle-treated rat brains. CONCLUSIONS: Epo exerts neuroprotective effect against traumatic brain injury via reducing Bax gene expression involved in inhibiting TBI-induced neuronal cell death. |
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Authors:
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Z B Liao; G Y Jiang; Z H Tang; X G Zhi; X C Sun; W Y Tang; M J Wu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neurology India Volume: 57 ISSN: 0028-3886 ISO Abbreviation: Neurol India Publication Date: 2009 Nov-Dec |
Date Detail:
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Created Date: 2010-02-08 Completed Date: 2010-04-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0042005 Medline TA: Neurol India Country: India |
Other Details:
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Languages: eng Pagination: 722-8 Citation Subset: IM |
Affiliation:
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Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. liaozhengbu@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Brain Injuries* / drug therapy, pathology, physiopathology Cell Survival / drug effects Cerebral Cortex / drug effects, pathology* Disease Models, Animal Down-Regulation / drug effects* Erythropoietin / pharmacology*, therapeutic use* Humans In Situ Nick-End Labeling / methods RNA, Messenger / metabolism Rats Rats, Wistar Time Factors bcl-2-Associated X Protein / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/bcl-2-Associated X Protein; 11096-26-7/Erythropoietin |
| Comments/Corrections | |
Comment In:
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Neurol India. 2009 Nov-Dec;57(6):693-4
[PMID:
20139494
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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