| Erythropoietin administration acutely stimulates resting energy expenditure in healthy young men. | |
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MedLine Citation:
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PMID: 22241056 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Treatment with recombinant human erythropoietin (rHuEpo) improves insulin sensitivity in patients with end-stage renal disease, and animal studies indicate that Epo increases fat oxidation. However, the metabolic effects of rHuEpo have never been experimentally studied in healthy humans. The aim was to investigate the effects of an acute rHuEpo bolus on substrate metabolism and insulin sensitivity in health young men. Ten healthy young men were studied in a single-blinded, randomized cross-over design with a 2-wk wash-out period receiving 400 IU/kg rHuEpo or placebo. Substrate metabolism was evaluated by indirect calorimetry and tracer infusions, insulin sensitivity by a hyperinsulinemic euglycemic clamp, and PCR and western blotting measured protein expression and content, respectively. Resting energy expenditure (REE) increased significantly after rHuEpo [Basal; 1863.3 ± 67.2 (kcal/day) (placebo) vs. 2041.6 ± 81.2 (rHuEpo) p<0.001, Clamp; 1903.9 ± 68.3 (placebo) vs. 2015.7 ± 114.4 (rHuEpo), p=0.03], but the increase could not be explained by changes in mRNA levels of uncoupling protein 2 or 3. Fat oxidation in the basal state tended to be higher after rHuEpo, but could not be explained by changes in mRNA levels of CPT1 and PPARα, or AMPK and ACC protein phosphorylation. Insulin stimulated glucose disposal, glucose metabolism, and whole-body and forearm protein metabolism did not change significantly in response to rHuEpo. In conclusion, a single injection of rHuEpo acutely increases REE in healthy human subjects. This calorigenic effect is not accompanied by distinct alterations in the pattern of substrate metabolism or insulin sensitivity. |
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Authors:
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Britt Christensen; Mikkel Holm Vendelbo; Thomas Krusenstjerna-Hafstrøm; Michael Madsen; Steen B Pedersen; Niels Jessen; Niels Moller; Jens Otto L Jorgensen |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-12 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: - ISSN: 1522-1601 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1Aarhus University. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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