Document Detail


Erythropoietic progenitor cells from premature neonates studied using a validated serum-deprived medium. Evidence that peripheral blood BFU-E from premature neonates are increased in number and are highly dependent upon burst promoting activity for in vitro growth.
MedLine Citation:
PMID:  1367667     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have examined a serum-deprived culture system in order to verify that it is suitable for the study of burst forming unit erythroid (BFU-E) progenitor cells from premature neonates. Optimum growth of BFU-E from premature neonates was observed with each media constituent using the same concentration as that previously described for adult subjects. Growth of immature BFU-E from premature neonates were highly dependent upon a source of Burst Promoting Activity and mature BFU-E derived colonies emerged at day 12 compared to day 14 in adults. Our preliminary results with the validated medium suggest that premature infants have increased peripheral blood concentrations of BFU-E compared to healthy adult controls.
Authors:
N B Westwood; A J Emmerson
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cytotechnology     Volume:  6     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1992-01-02     Completed Date:  1992-01-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  241-6     Citation Subset:  B    
Affiliation:
Division of Haematology, United Medical School of Guy's Hospital, University of London, U.K.
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MeSH Terms
Descriptor/Qualifier:
Adult
Cell Differentiation
Cells, Cultured
Culture Media, Serum-Free*
Erythroid Precursor Cells / metabolism*
Erythropoietin / pharmacology
Gestational Age
Humans
Immunophenotyping
Infant
Infant, Newborn
Infant, Premature / blood
Interleukin-3 / pharmacology
Middle Aged
Chemical
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/Interleukin-3; 11096-26-7/Erythropoietin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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