Document Detail


Erythromycin modulates IL-8 expression in normal and inflamed human bronchial epithelial cells.
MedLine Citation:
PMID:  9230759     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Erythromycin (EM) and its 14-member macrolide analogues have attracted attention for its effectiveness in a variety of airway diseases, including diffuse panbronchiolitis (DPB), sinobronchial syndrome, and chronic sinusitis. However, its mechanisms of action remain unelucidated. We evaluated the effects of several antibiotics on IL-8 expression by normal and transformed human bronchial epithelial cells, an important source of this potent chemokine involved in cell recruitment into the airways. EM and clarithromycin (CAM) uniquely suppressed mRNA levels as well as the release of IL-8 at the therapeutic and noncytotoxic concentrations (% inhibition of IL-8 protein release: 25.0 +/- 5.67% and 37.5 +/- 8.99%, respectively, at 10(-6) M). The other antimicrobes, including a 16-member macrolide josamycin, showed no effect. Bronchial epithelial cells from very peripheral airways as well as from main bronchi were obtained from patients with chronic airway inflammatory diseases, and EM and CAM inhibited IL-8 release from these cells. Among five patients who underwent bronchoscopy before and after macrolide treatment, four showed decreased levels of IL-8 expression in airway epithelium as assessed by reverse transcription and polymerase chain reaction. Our findings showed these 14-member macrolides had inhibitory effect on IL-8 expression in human bronchial epithelial cells, and this new mode of action may have relevance to their clinical effectiveness in airway diseases.
Authors:
H Takizawa; M Desaki; T Ohtoshi; S Kawasaki; T Kohyama; M Sato; M Tanaka; T Kasama; K Kobayashi; J Nakajima; K Ito
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  156     ISSN:  1073-449X     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-08-12     Completed Date:  1997-08-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  266-71     Citation Subset:  AIM; IM    
Affiliation:
Department of Medicine and Physical Therapy, University of Tokyo, School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / pharmacology*
Bronchi / drug effects*,  immunology*
Cells, Cultured
Epithelial Cells
Epithelium / drug effects,  immunology
Erythromycin / pharmacology*
Humans
Interleukin-8 / analysis*,  genetics
RNA, Messenger / genetics
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Interleukin-8; 0/RNA, Messenger; 114-07-8/Erythromycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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