Document Detail

Erythromycin modulates IL-8 expression in normal and inflamed human bronchial epithelial cells.
MedLine Citation:
PMID:  9230759     Owner:  NLM     Status:  MEDLINE    
Erythromycin (EM) and its 14-member macrolide analogues have attracted attention for its effectiveness in a variety of airway diseases, including diffuse panbronchiolitis (DPB), sinobronchial syndrome, and chronic sinusitis. However, its mechanisms of action remain unelucidated. We evaluated the effects of several antibiotics on IL-8 expression by normal and transformed human bronchial epithelial cells, an important source of this potent chemokine involved in cell recruitment into the airways. EM and clarithromycin (CAM) uniquely suppressed mRNA levels as well as the release of IL-8 at the therapeutic and noncytotoxic concentrations (% inhibition of IL-8 protein release: 25.0 +/- 5.67% and 37.5 +/- 8.99%, respectively, at 10(-6) M). The other antimicrobes, including a 16-member macrolide josamycin, showed no effect. Bronchial epithelial cells from very peripheral airways as well as from main bronchi were obtained from patients with chronic airway inflammatory diseases, and EM and CAM inhibited IL-8 release from these cells. Among five patients who underwent bronchoscopy before and after macrolide treatment, four showed decreased levels of IL-8 expression in airway epithelium as assessed by reverse transcription and polymerase chain reaction. Our findings showed these 14-member macrolides had inhibitory effect on IL-8 expression in human bronchial epithelial cells, and this new mode of action may have relevance to their clinical effectiveness in airway diseases.
H Takizawa; M Desaki; T Ohtoshi; S Kawasaki; T Kohyama; M Sato; M Tanaka; T Kasama; K Kobayashi; J Nakajima; K Ito
Related Documents :
18566469 - Functions of the type 1 bmp receptor acvr1 (alk2) in lens development: cell proliferati...
3207189 - Electron microscopic studies on the thymus of the arabian camel (camelus dromedarius).
15752729 - Functional characterization of a conditionally immortalized mouse epididymis caput epit...
6189269 - Demonstration of keratin filaments in thymic nurse cells (tnc) and alloreactivity of tn...
20118219 - Temporal relationship between primary and motile ciliogenesis in airway epithelial cells.
196249 - Epithelial cells and von gierke's disease.
9583789 - Changes in mucosal goblet cell density in acute otitis media caused by non-typeable hae...
10771309 - Diminished prohormone convertase 3 expression (pc1/pc3) inhibits progastrin post-transl...
23662679 - Timely expression of the arabidopsis stoma-fate master regulator mute is required for s...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  156     ISSN:  1073-449X     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-08-12     Completed Date:  1997-08-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  266-71     Citation Subset:  AIM; IM    
Department of Medicine and Physical Therapy, University of Tokyo, School of Medicine, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Anti-Bacterial Agents / pharmacology*
Bronchi / drug effects*,  immunology*
Cells, Cultured
Epithelial Cells
Epithelium / drug effects,  immunology
Erythromycin / pharmacology*
Interleukin-8 / analysis*,  genetics
RNA, Messenger / genetics
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Interleukin-8; 0/RNA, Messenger; 114-07-8/Erythromycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effects of neutrophil elastase inhibitor on bleomycin-induced pulmonary fibrosis in mice.
Next Document:  Intraalveolar expression of tumor necrosis factor-alpha gene during conventional and high-frequency ...