Document Detail


Erythrocytes protect alpha-1-proteinase inhibitor from oxidative inactivation induced by chemicals, the myeloperoxidase-H2O2-halide system and stimulated polymorphonuclear leukocytes.
MedLine Citation:
PMID:  1652455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The oxidative inactivation of the alpha-1-proteinase inhibitor (alpha 1PI) is one of the mechanisms responsible for creating the elastase/antielastase imbalance during inflammation in the lower respiratory tract. Chronic supremacy of elastase may cause degradation of elastin fibers leading to the pulmonary emphysema. In this study we have investigated the effect of erythrocytes (RBC, concentrations 0.125 to 1.5%) on the oxidative inactivation of alpha 1PI by the phorbol myristate acetate-stimulated polymorphonuclear leukocytes (PMNL) and PMNL myeloperoxidase-H2O2-halide system. During exposure of alpha 1PI to both systems in the presence of RBC the significant protection (p less than 0.001) of alpha 1PI was found for all RBC concentrations, e.g. at RBC concentration of 1% the elastase inhibitory capacity (EIC) of alpha 1PI increased from 0 to 60 +/- 6 and 88 +/- 9% of the control value (untreated alpha 1PI), n = 5, respectively. The preincubation of RBC with chloramine-T (1 mM), inhibition of RBC catalase or depletion of RBC reduced glutathione alone did not diminish the capacity of RBC to protect alpha 1PI. However, these treatments together completely deprived RBC of their protective properties. Moreover, we have compared the decrease in the EIC of human blood and its plasma after incubation with H2O2 (0.1 mM to 0.1 M) or chloramine-T (1 microM to 1 mM). For the incubation with H2O2 no decrease in blood EIC was found whereas in plasma the loss of EIC was already visible at a H2O2 concentration of 0.1 mM. Also for the incubation with chloramine-T the EIC of blood was more resistant to oxidant damage than EIC of plasma. It is suggested that RBC contaminations present in the phagocyte inflammatory infiltration in the lower airways may protect alpha 1PI from oxidative inactivation and thus indirectly diminish proteolytic lung injury related to inflammation.
Authors:
D Nowak; G Piasecka
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Experimental pathology     Volume:  42     ISSN:  0232-1513     ISO Abbreviation:  Exp Pathol     Publication Date:  1991  
Date Detail:
Created Date:  1991-10-03     Completed Date:  1991-10-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8108218     Medline TA:  Exp Pathol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  47-58     Citation Subset:  IM    
Affiliation:
Department of Pneumology and Allergology, Medical Academy, Lódź, Poland.
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MeSH Terms
Descriptor/Qualifier:
Chloramines / pharmacology
Erythrocytes / physiology*
Humans
Hydrogen Peroxide / pharmacology*
Neutrophils / physiology*
Oxidation-Reduction
Pancreatic Elastase / metabolism,  physiology
Peroxidase / pharmacology*
Tetradecanoylphorbol Acetate / pharmacology
alpha 1-Antitrypsin / metabolism*
Chemical
Reg. No./Substance:
0/Chloramines; 0/alpha 1-Antitrypsin; 10599-90-3/chloramine; 16561-29-8/Tetradecanoylphorbol Acetate; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.7/Peroxidase; EC 3.4.21.36/Pancreatic Elastase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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