Document Detail

Erythrocyte mechanical fragility is increased in patients with type 2 diabetes.
MedLine Citation:
PMID:  22284245     Owner:  NLM     Status:  In-Data-Review    
BACKGROUND: Anemia is common among patients with type 2 diabetes. We determined whether type 2 diabetic patients significantly differed in erythrocyte mechanical fragility as compared with nondiabetic subjects.
METHODS: We recruited 25 Caucasian patients with type 2 diabetes (14 men and 11 women; mean age 58±8years) and 25 age-, race- and sex-matched nondiabetic individuals. The fragility of erythrocytes was tested by inducing mechanical hemolysis by double aspiration of K(2)EDTA blood through a 0.5mL insulin syringe equipped with a very thin needle. The plasma was then separated from the blood cells by centrifugation at 2000xg for 15min at room temperature. A Beckman Coulter DxC 800 was used to measure the hemolysis index by direct spectrophotometry.
RESULTS: Compared with matched nondiabetic controls, type 2 diabetic patients had a significantly increased mechanical fragility of erythrocytes (hemolysis index ratio 21±13 vs. 14±10, p=0.02). Univariable linear regression analysis revealed that there was a strong positive association between percent hemolysis and fasting plasma glucose (r=0.669, p<0.001) and hemoglobin A1c (r=0.549, p<0.005) in type 2 diabetic subjects, but not in matched nondiabetic controls.
CONCLUSIONS: Our data suggest that patients with type 2 diabetes have a significantly higher erythrocyte mechanical fragility than matched nondiabetic subjects, and that fasting plasma glucose is the strongest correlate of increased mechanical fragility of erythrocytes in this patients group.
Giuseppe Lippi; Mariella Mercadanti; Rosalia Aloe; Giovanni Targher
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Publication Detail:
Type:  Journal Article     Date:  2011-11-30
Journal Detail:
Title:  European journal of internal medicine     Volume:  23     ISSN:  1879-0828     ISO Abbreviation:  Eur. J. Intern. Med.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-01-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9003220     Medline TA:  Eur J Intern Med     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  150-3     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 European Federation of Internal Medicine. Published by Elsevier B.V. All rights reserved.
Clinical Chemistry and Hematology Laboratory, Department of Pathology and Laboratory Medicine, Academic Hospital of Parma, Parma, Italy.
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