Document Detail

Erythrocyte incorporation of iron by infants: iron bioavailability from a low-iron infant formula and an evaluation of the usefulness of correcting erythrocyte incorporation values, using a reference dose or plasma ferritin concentrations.
MedLine Citation:
PMID:  11177201     Owner:  NLM     Status:  MEDLINE    
Bioavailability of iron (Fe) from a low-Fe infant formula was determined by erythrocyte incorporation of 58Fe 14 d after administration in ten healthy, non-Fe-deficient infants. Two feeding protocols were compared, with each infant acting as his/her own control. At 140 and 154 d of age, infants were fed 1000 g of 58Fe-labelled formula (1.44 mg total Fe/1000 g) as six feeds over 24 h (Protocol A) or as two feeds/day on three consecutive days (Protocol B). A water solution with 57Fe and ascorbic acid was given separately as a reference dose in both study protocols. Erythrocyte incorporation of 58Fe and 57Fe was determined by thermal ionisation mass spectrometry. Geometric mean 58Fe incorporation was 7.6% (range 3.3-13.5%) with Protocol A as compared to 10.6% (range 6.7-18.6%) with Protocol B (P = 0.05); paired t test. Inter-individual variability of 58Fe was not reduced by correcting for the incorporation of 57Fe from the reference dose, or by correcting for plasma ferritin concentration. Fractional erythrocyte incorporation of Fe from low-Fe infant formula was in the same range as our earlier published data on erythrocyte incorporation of Fe from human milk extrinsically labelled with 58Fe (Davidsson et al. 1994a). The methodological evaluations included in this study clearly indicate the importance of using standardised study protocols when evaluating Fe bioavailability in infants. Corrections of erythrocyte incorporation data based on plasma ferritin or erythrocyte incorporation of Fe from a reference dose were not found to be useful.
L Davidsson; E E Ziegler; P Kastenmayer; R F Hurrell
Publication Detail:
Type:  Clinical Trial; Evaluation Studies; Journal Article; Randomized Controlled Trial    
Journal Detail:
Title:  The British journal of nutrition     Volume:  84     ISSN:  0007-1145     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-03-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  847-53     Citation Subset:  IM    
Nestlé Research Center Lausanne, PO Box 44, 1000 Lausanne 26, Switzerland.
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MeSH Terms
Biological Availability
Body Weight
Erythrocytes / metabolism*
Ferritins / blood*
Infant Food / analysis*
Infant Nutritional Physiological Phenomena / physiology*
Iron Isotopes / diagnostic use
Iron, Dietary / blood*
Reference Standards
Reg. No./Substance:
0/Iron Isotopes; 0/Iron, Dietary; 9007-73-2/Ferritins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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