Document Detail


Erythrocyte heterogeneity in sickle cell disease: effect of deoxygenation on intracellular polymer formation and rheology of sub-populations.
MedLine Citation:
PMID:  2757967     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Erythrocytes from 12 patients with homozygous sickle cell disease in the steady state were fractionated on a Percoll-Stractan density gradient. Erythrocyte deformability was measured by initial-flow-rate filtration through pores of 5 microns diameter and erythrocyte polymer content was calculated as a function of oxygen saturation. Density fractionated sub-populations of sickle cells showed distinct rheological characteristics, the filterability of dense cells being impaired by minimal oxygen desaturation with the apparent formation of little or no intracellular polymer. Lighter cell fractions required a greater degree of deoxygenation and polymer formation to impair deformability, although this occurred prior to morphological sickling. Dense cells therefore exert a disproportionate effect on blood rheology in sickle cell disease and are likely to have an adverse rheological effect in vivo at arterial oxygen tension.
Authors:
A J Keidan; C T Noguchi; M Player; S M Chalder; J Stuart
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of haematology     Volume:  72     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-09-18     Completed Date:  1989-09-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  254-9     Citation Subset:  IM    
Affiliation:
Department of Haematology, Medical School, University of Birmingham, U.K.
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MeSH Terms
Descriptor/Qualifier:
Anemia, Sickle Cell / blood*
Erythrocyte Deformability / drug effects
Erythrocytes, Abnormal / physiology*
Humans
Oxygen / pharmacology*
Polymers / metabolism
Rheology
Chemical
Reg. No./Substance:
0/Polymers; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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