Document Detail

Errors in estimating neonatal production of glucose with stable isotopes during "approximate steady state".
MedLine Citation:
PMID:  11768326     Owner:  NLM     Status:  MEDLINE    
In studies of glucose metabolism in neonates, a commonly used approach is based on constant rate infusion of glucose labelled with a stable isotope in order to reach an approximate steady state with regard to isotopic enrichment in plasma. Under presumed conditions of a glucose steady state, the rates of appearance and disappearance of glucose can, in principle, be calculated with use of a simple steady-state formula. However, in the neonate, steady-state conditions do not generally prevail and results derived on such assumptions are questionable. In the present study, we have taken a pragmatic approach and compared estimates obtained from the conventional formula with values calculated with Steele's non-steady-state method. The results show that the estimated glucose appearance and disappearance rates change more or less over the observed time period in all the typical cases studied, and that "steady-state estimates" may differ from the corresponding non-steady-state values by up to 37%. In a sensitivity analysis, the value of the distribution volume factor was found to be non-critical, a circumstance that supports the use of Steele's method. Thus, even though the classical Steele pool-fraction method for computation of rates of appearance and disappearance under non-steady-state conditions has been criticized, it is still the most realistic alternative to the frequently used simple steady-state formula in applications to newborns.
T Groth; U Ewald; J Gustafsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of clinical and laboratory investigation     Volume:  61     ISSN:  0036-5513     ISO Abbreviation:  Scand. J. Clin. Lab. Invest.     Publication Date:  2001  
Date Detail:
Created Date:  2001-12-20     Completed Date:  2002-05-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404375     Medline TA:  Scand J Clin Lab Invest     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  663-79     Citation Subset:  IM    
Unit for Biomedical Informatics and Systems Analysis, Department of Medical Sciences, Uppsala University, Sweden.
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MeSH Terms
Deuterium / diagnostic use
Glucose / biosynthesis*,  pharmacokinetics*
Infant, Newborn
Infant, Premature / metabolism*
Models, Biological*
Neonatal Screening / methods
Sensitivity and Specificity
Reg. No./Substance:
50-99-7/Glucose; 7782-39-0/Deuterium

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