| Error-prone mutagenesis detected in mammalian cells by a shuttle vector containing the supF gene of Escherichia coli. | |
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MedLine Citation:
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PMID: 6095049 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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When a shuttle vector containing a tyrosine suppressor tRNA (supF) gene as a target for mutagenesis replicated in a monkey kidney cell line, the frequency of SupF+ mutations was 2.3 +/- 0.5 x 10(-3). When the host cells were treated with ethyl methanesulfonate 40 h before transfection, a 10-fold increase in SupF+ mutation frequency was observed. These results supported the hypothesis that a damage-inducible mutagenic pathway exists in mammalian cells and also demonstrated the utility of this shuttle vector for the study of mutagenesis in mammalian cells. |
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Authors:
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S Sarkar; U B Dasgupta; W C Summers |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular biology Volume: 4 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1984 Oct |
Date Detail:
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Created Date: 1985-01-10 Completed Date: 1985-01-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2227-30 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cercopithecus aethiops Escherichia coli / genetics* Ethyl Methanesulfonate / pharmacology Genetic Vectors* Mutation* Plasmids Time Factors Transfection |
| Grant Support | |
ID/Acronym/Agency:
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CA 06519/CA/NCI NIH HHS; CA 13515/CA/NCI NIH HHS; CA 16038/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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62-50-0/Ethyl Methanesulfonate |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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