Document Detail


Error-prone mutagenesis detected in mammalian cells by a shuttle vector containing the supF gene of Escherichia coli.
MedLine Citation:
PMID:  6095049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
When a shuttle vector containing a tyrosine suppressor tRNA (supF) gene as a target for mutagenesis replicated in a monkey kidney cell line, the frequency of SupF+ mutations was 2.3 +/- 0.5 x 10(-3). When the host cells were treated with ethyl methanesulfonate 40 h before transfection, a 10-fold increase in SupF+ mutation frequency was observed. These results supported the hypothesis that a damage-inducible mutagenic pathway exists in mammalian cells and also demonstrated the utility of this shuttle vector for the study of mutagenesis in mammalian cells.
Authors:
S Sarkar; U B Dasgupta; W C Summers
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  4     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1985-01-10     Completed Date:  1985-01-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2227-30     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cercopithecus aethiops
Escherichia coli / genetics*
Ethyl Methanesulfonate / pharmacology
Genetic Vectors*
Mutation*
Plasmids
Time Factors
Transfection
Grant Support
ID/Acronym/Agency:
CA 06519/CA/NCI NIH HHS; CA 13515/CA/NCI NIH HHS; CA 16038/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
62-50-0/Ethyl Methanesulfonate
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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