| Erk is involved in the differentiation induced by diallyl disulfide in the human gastric cancer cell line MGC803. | |
MedLine Citation:
|
PMID: 16874458 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Diallyl disulfide (DADS) is a major constituent of garlic. Previously, we found that DADS both inhibited proliferation in human gastric cancer cells in vitro and in vivo, and induced G2/M arrest. In this study, we investigated whether this differentiation effect was induced by DADS in human gastric cancer MGC803 cells, and whether it was related to an alteration in ERK activity. The results showed that the growth of MGC803 cells was inhibited by DADS. Cells treated with DADS displayed a lower nucleocytoplasmic ratio and tended to form gland and intercellular conjunction structures. The ConA-mediated cell agglutination ratio and cells' ALP specific activity decreased. In MGC803 cells, dye transfer was limited to a few cells neighbouring the dye-injected cell and to a depth of 1-2 layers beneath the scrape site. However, after treatment with DADS, the LY (Lucifer Yellow) was transferred to several cells immediately neighbouring the microinjected cell and to a depth of 2-4 cell layers from the scrape site. This indicated that DADS induced differentiation in MGC803 cells. Western blot analysis revealed that although DADS did not influence the quantity of ERK1/2 protein expressed, it did decrease its phosphorylation in a concentration-dependent manner, compared with the controls. At 30 mg x L(-1), DADS inhibited the activation of ERK1/2 in 15-30 min. These results suggested that the DADS-induced differentiation of MGC803 cells involved an alteration of the ERK1/2 signaling pathway. |
Authors:
|
Hui Ling; Liang-Yun Zhang; Qi Su; Ying Song; Zhao-Yang Luo; Xiu Tian Zhou; Xi Zeng; Jie He; Hui Tan; Jing-Ping Yuan |
Related Documents
:
|
2672658 - An immunohistochemical study on the distribution of endocrine cells in the gastrointest... 22678878 - A dual gradient assay for the parametric analysis of cell-surface interactions. 10811788 - Megagametophyte development in potentilla nivea (rosaceae) from northern swedish lapland. 2736608 - Quantitative electron microscopy of endocrine cells in oxyntic mucosa of normal human s... 19778378 - U2os cells lacking chk1 undergo aberrant mitosis and fail to activate the spindle check... 25421758 - Efficient single-cell poration by microsecond laser pulses. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Cellular & molecular biology letters Volume: 11 ISSN: 1689-1392 ISO Abbreviation: Cell. Mol. Biol. Lett. Publication Date: 2006 |
Date Detail:
|
Created Date: 2006-08-15 Completed Date: 2007-09-06 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 9607427 Medline TA: Cell Mol Biol Lett Country: Poland |
Other Details:
|
Languages: eng Pagination: 408-23 Citation Subset: IM |
Affiliation:
|
Cancer Research Institute, Nanhua University, Hengyang City, Hunan Province, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Agglutination
/
drug effects Alkaline Phosphatase / metabolism Allyl Compounds / pharmacology* Butadienes / pharmacology Cell Differentiation / drug effects* Cell Line, Tumor Cell Survival / drug effects Concanavalin A / pharmacology Disulfides / pharmacology* Humans Mitogen-Activated Protein Kinase 1 / metabolism* Mitogen-Activated Protein Kinase 3 / metabolism* Nitriles / pharmacology Phosphoproteins / metabolism Phosphorylation / drug effects Stomach Neoplasms / enzymology*, pathology*, ultrastructure |
| Chemical | |
Reg. No./Substance:
|
0/Allyl Compounds; 0/Butadienes; 0/Disulfides; 0/Nitriles; 0/Phosphoproteins; 0/U 0126; 11028-71-0/Concanavalin A; 2179-57-9/diallyl disulfide; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Enhanced expression of selenocysteine lyase in acute glomerulonephritis and its regulation by AP-1.
Next Document: The atypical pattern of cell death in B16F10 melanoma cells treated with TNP-470.