Document Detail

Erk is involved in the differentiation induced by diallyl disulfide in the human gastric cancer cell line MGC803.
MedLine Citation:
PMID:  16874458     Owner:  NLM     Status:  MEDLINE    
Diallyl disulfide (DADS) is a major constituent of garlic. Previously, we found that DADS both inhibited proliferation in human gastric cancer cells in vitro and in vivo, and induced G2/M arrest. In this study, we investigated whether this differentiation effect was induced by DADS in human gastric cancer MGC803 cells, and whether it was related to an alteration in ERK activity. The results showed that the growth of MGC803 cells was inhibited by DADS. Cells treated with DADS displayed a lower nucleocytoplasmic ratio and tended to form gland and intercellular conjunction structures. The ConA-mediated cell agglutination ratio and cells' ALP specific activity decreased. In MGC803 cells, dye transfer was limited to a few cells neighbouring the dye-injected cell and to a depth of 1-2 layers beneath the scrape site. However, after treatment with DADS, the LY (Lucifer Yellow) was transferred to several cells immediately neighbouring the microinjected cell and to a depth of 2-4 cell layers from the scrape site. This indicated that DADS induced differentiation in MGC803 cells. Western blot analysis revealed that although DADS did not influence the quantity of ERK1/2 protein expressed, it did decrease its phosphorylation in a concentration-dependent manner, compared with the controls. At 30 mg x L(-1), DADS inhibited the activation of ERK1/2 in 15-30 min. These results suggested that the DADS-induced differentiation of MGC803 cells involved an alteration of the ERK1/2 signaling pathway.
Hui Ling; Liang-Yun Zhang; Qi Su; Ying Song; Zhao-Yang Luo; Xiu Tian Zhou; Xi Zeng; Jie He; Hui Tan; Jing-Ping Yuan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular & molecular biology letters     Volume:  11     ISSN:  1689-1392     ISO Abbreviation:  Cell. Mol. Biol. Lett.     Publication Date:  2006  
Date Detail:
Created Date:  2006-08-15     Completed Date:  2007-09-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9607427     Medline TA:  Cell Mol Biol Lett     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  408-23     Citation Subset:  IM    
Cancer Research Institute, Nanhua University, Hengyang City, Hunan Province, China.
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MeSH Terms
Agglutination / drug effects
Alkaline Phosphatase / metabolism
Allyl Compounds / pharmacology*
Butadienes / pharmacology
Cell Differentiation / drug effects*
Cell Line, Tumor
Cell Survival / drug effects
Concanavalin A / pharmacology
Disulfides / pharmacology*
Mitogen-Activated Protein Kinase 1 / metabolism*
Mitogen-Activated Protein Kinase 3 / metabolism*
Nitriles / pharmacology
Phosphoproteins / metabolism
Phosphorylation / drug effects
Stomach Neoplasms / enzymology*,  pathology*,  ultrastructure
Reg. No./Substance:
0/Allyl Compounds; 0/Butadienes; 0/Disulfides; 0/Nitriles; 0/Phosphoproteins; 0/U 0126; 11028-71-0/Concanavalin A; 2179-57-9/diallyl disulfide; EC Protein Kinase 1; EC Protein Kinase 3; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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